Brevibacillus panacihumi sp. nov., a {beta}-glucosidase-producing bacterium

1 Korean Ginseng Center and Ginseng Genetic Resource Bank, Kyung Hee University, 1 Seocheon-dong, Giheung-gu Yongin-si, Gyeonggi-do 449-701, Republic of Korea 2 Department of Bio & Environmental Technology, Division of Environmental & Life Science, College of Natural Science, Seoul Women's University, 623 Hwarangno, Nowon-gu, Seoul 139-774, Republic of Korea Correspondence Deok-Chun Yang dcyang{at}khu.ac.kr Two Gram-positive-staining, endospore-forming, rod-shaped, motile bacteria, strains DCY35 T and C17, were isolated from soil of a ginseng field in South Korea and were characterized in order to determine their taxonomic positions. 16S rRNA gene sequence analysis revealed that the two strains belonged to the family Paenibacillaceae ; strain DCY35 T showed highest levels of similarity to strain C17 (99.9 %), Brevibacillus invocatus LMG 18962 T (98.9 %), B. centrosporus DSM 8445 T (98.0 %), B. borstelensis DSM 6347 T (97.6 %), B. formosus DSM 9885 T (97.4 %), B. agri DSM 6348 T (97.3 %), B. brevis DSM 30 T (97.3 %) and B. levickii LMG 22481 T (97.0 %). Chemotaxonomic analyses revealed that strains DCY35 T and C17 possess menaquinone MK-7, common to members of the genus Brevibacillus , and that the predominant fatty acids were iso-C 15 : 0 (37.3 % of the total), anteiso-C 15 : 0 (32.9 %), iso-C 14 : 0 (11.8 %) and iso-C 16 : 0 (6.5 %). The results of physiological and biochemical tests clearly demonstrated that strains DCY35 T and C17 represent a distinct species. Based on these data, the two strains are considered to represent a novel species of the genus Brevibacillus , for which the name Brevibacillus panacihumi sp. nov. is proposed. The type strain is DCY35 T (=KCTC 13206 T =JCM 15085 T ). These authors contributed equally to this work. The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA gene sequences of strains DCY35 T and C17 are EU383033 and EU383032 , respectively. A table giving levels of DNA–DNA relatedness among strains DCY35 T and C17 and the type strains of eight closely related Brevibacillus species is available with the online version of this paper.