Effects of scutellarin on PKCγ in PC12 cell injury induced by oxygen and glucose deprivation

Aim: To evaluate the neuroprotective effect and mechanisms of scutellarin (Scu) against PC12 cell injury after oxygen and glucose deprivation followed by reperfusion (OGD-Rep). Methods: Undifferentiated rat pheochromocytoma PC12 cells, exposed to oxygen and glucose deprivation followed by reperfusio...

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Veröffentlicht in:Acta pharmacologica Sinica 2007-10, Vol.28 (10), p.1573-1579
Hauptverfasser: XU, Wei, ZHA, Ruo‐peng, WANG, Wen‐yi, WANG, Yi‐ping
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Sprache:eng
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Zusammenfassung:Aim: To evaluate the neuroprotective effect and mechanisms of scutellarin (Scu) against PC12 cell injury after oxygen and glucose deprivation followed by reperfusion (OGD-Rep). Methods: Undifferentiated rat pheochromocytoma PC12 cells, exposed to oxygen and glucose deprivation followed by reperfusion (OGD- Rep), used as an in vitro model of ischemia/reperfusion. Cell survival was evaluated by diphenyltetrazolium bromide (MTT) assay and the amount of LDH release was determined using assay kits. [Ca^2+]i was monitored using a fluorescent Ca^2+ - sensitive dye Fura-2 acetoxymethyl ester. Cell apoptosis was detected by a DNA ladder and by flow cytometric detection. The expression of protein kinase C (PKC)T was determined usiiag both RT-PCR and Western blotting. The translocation of PKCT was assayed by subcellular fractionation and Western blotting. Results: OGD-Rep injury significantly elevated the level of LDH release, [Ca^2+]i, mRNA expression and the translocation of PKCγ compared in the PC12 cells with those of the normal group. Scu (10-100 μmol/L) exerted a protective effect against OGD-Rep injury by reducing LDH release, [Ca^2+]i, the percent of apoptosis, and the translocation of PKCγ. Conclusion: Scu inhibits the increase of [Ca^2+]i and the activation of PKCγ, exerting protective effects against PC12 cell injury induced by OGD-Rep.
ISSN:1671-4083
1745-7254
DOI:10.1111/j.1745-7254.2007.00502.x