Environmental causes and transgenerational consequences of ecdysteroid hormone provisioning in Acheta domesticus

[Display omitted] •Variation in egg 20-hydroxyecdysone alters offspring phenotype in Acheta domesticus.•20-hydroxyecdysone deposition to eggs reflects the grandmaternal environment.•Maternal and grandmaternal environments interact to influence female fecundity. An animal’s phenotype may be shaped by its genes, but also reflects its own environment and often that of its parents. Nongenetic parental effects are often mediated by steroid hormones, and operate between parents and offspring through mechanisms that are well described in vertebrate and model systems. However, less is understood about the strength and frequency of hormone mediated nongenetic parental effects across more than one generation of descendants, and in nonmodel systems. Here we show that the concentration of active ecdysteroid hormones provided by a female house cricket (Acheta domesticus) affects the growth rate of her offspring. We also reveal that variation in the active ecdysteroid hormones provided by a female house cricket to her eggs derives primarily from the quality of nutrition available to her maternal grandmother, regardless of genetic background. This finding is in stark contrast to most previous work that documents a decline in the strength of environmentally based parental effects with each passing generation. Strong grandparental effects may be adaptive under predictable, cyclical changes in the environment. Our results also suggest that hormone-mediated grand-maternal effects represent an important potential mechanism by which organisms can respond to environmental variability, and that further study of hormone-mediated carryover effects in this context could be profitable.