Live bird markets in Bangladesh as a potentially important source for Avian Influenza Virus transmission
•Multi-year active surveillance of AIV in LBM birds and peri-domestic wild birds near those 10 selected LBM of Bangladesh.•Used rt-PCR for detection of the AIV M-gene and AIV subtypes H5, H7 and H9 from LBM birds swab samples, and c-ELISA for AIV antibody detection from serum samples of wild birds.•...
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Veröffentlicht in: | Preventive veterinary medicine 2018-08, Vol.156, p.22-27 |
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Zusammenfassung: | •Multi-year active surveillance of AIV in LBM birds and peri-domestic wild birds near those 10 selected LBM of Bangladesh.•Used rt-PCR for detection of the AIV M-gene and AIV subtypes H5, H7 and H9 from LBM birds swab samples, and c-ELISA for AIV antibody detection from serum samples of wild birds.•Found significantly higher AIV prevalence in winter compared to summer and a similar pattern was found in AIV antibody prevalence in wild birds feeding in the direct vicinity of LBM.•Considerable exchange of AIV within and among the four LBM bird species and wild birds due to poor biosecurity.•Found a significantly higher proportion of AIV H5 in LBM chickens and H9 in LBM ducks.•No H7 was detected in any of the investigated samples.
Live bird markets (LBM) are important for trading poultry in many developing countries where they are being considered hotspots of Avian Influenza Virus (AIV) prevalence and contamination. An active surveillance for Avian Influenza Virus (AIV) was conducted on four species of LBM birds (chickens, ducks, quails and pigeons) from 10 of the largest LBM in Chittagong, Bangladesh, and two species of peri-domestic wild birds (house crow and Asian pied starling) in their direct vicinity from November 2012 until September 2016. Our aim was to identify the scale and annual pattern of AIV circulation in both the LBM birds and the two per-domestic wild bird species living in close proximity of the LBM. In the latter two species, the annual pattern in AIV antibody prevalence was additionally investigated. A total of 4770 LBM birds and 1119 peri-domestic wild birds were sampled. We used rt-PCR for detection of the AIV M-gene and AIV subtypes H5, H7 and H9 from swab samples. We used c-ELISA for AIV antibody detection from serum samples of peri-domestic wild birds. Average AIV prevalence among the four LBM species varied between 16 and 28%, whereas no AIV was detected in peri-domestic wild birds by rt-PCR. In all LBM species we found significantly higher AIV prevalence in winter compared to summer. A similar pattern was found in AIV antibody prevalence in peri-domestic wild birds feeding in the direct vicinity of LBM. For the subtypes of AIV investigated, we found a significantly higher proportion of AIV H5 in LBM chickens and H9 in LBM ducks. No H7 was detected in any of the investigated samples. We conclude that AIV and notably AIV H5 and H9 were circulating in the investigated LBM of Bangladesh with clear seasonality that matched the prevalence |
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ISSN: | 0167-5877 1873-1716 |
DOI: | 10.1016/j.prevetmed.2018.05.003 |