Paternity testing of wild black rockfish Sebastes inermis (Brownish type) from the Seto Inland Sea of Japan

We developed four microsatellite DNA loci to test for multiple paternity of black rockfish, Sebastes inermis, from the Seto Inland Sea of Japan. All loci showed a high degree of polymorphism (number of alleles per locus = 10 to 14, expected heterozygosity = 0.80) and discriminating power (probability of identity index = 3.71 x 10E-6, exclusion probability = 0.999) in unrelated wild specimens (n = 32). Genotypic assignment of five dams (109-220 mm in total length) and 50 embryos from each dam (n = 50) indicated that four dams were mated with a single sire. Only for one dam and three of her embryos we could not exclude multiple paternity.