Bacterial thermostable b-glucanases as a tool for plant functional genomics

A new strategy for creating experimental models for functional genomics has been proposed. It is based on the expression in transgenic plants of genes from thermophilic bacteria encoding functional analogues of plant proteins with high specific activity and thermal stability. We have validated this strategy by comparing physiological, biochemical and molecular properties of control tobacco plants and transgenic plants expressing genes of b-glucanases with different substrate specificity. We demonstrate that the expression of bacterial b-1,3-1,4-glucanase gene exerts no significant influence on tobacco plant metabolism, while the expression of bacterial b-1,3-glucanase affects plant metabolism only at early stages of growth and development. By contrast, the expression of bacterial b-1,4-glucanase has a significant effect on transgenic tobacco plant metabolism, namely, it affects plant morphology, the thickness of the primary cell wall, phytohormonal status, and the relative sugar content. We propose a hypothesis of b-glucanase action as an important factor of genetic regulation of metabolic processes in plants. mosaic virus (lichenase) under control of the 35S RNA CaMV promoter under control of the 35S RNA CaMV promoter (laminarinase) under control of the 35S RNA CaMV promoter the 35S RNA CaMV promoter and fused at their N-ends with the leader signal of carrot extensin which ensures secretion of proteins into the apoplast provides kanamicine resistance