Characterization and resuscitation of viable but nonculturable Vibrio alginolyticus VIB283

The aim of this study was to investigate the viable but nonculturable (VBNC) state of the bacterium. Vibrio alginolyticus VIB283 was cultured in sterilized seawater microcosm at 4°C. Culturability of the cells in the microcosm was monitored by spread plate count (PC) on 2216E agar, PCs declined to u...

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Veröffentlicht in:	Archives of microbiology 2007-09, Vol.188 (3), p.283-288
Hauptverfasser:	Du, Meng, Chen, Jixiang, Zhang, Xiaohua, Li, Aijuan, Li, Yun
Format:	Artikel
Sprache:	eng
Schlagworte:	Acridine Orange - analysis Animals Bacteria Bacteriological Techniques - methods Bacteriology Biological and medical sciences Chemical analysis Chick Embryo flow cytometry Fundamental and applied biological sciences. Psychology Marine Microbial Viability Microbiology Miscellaneous Resuscitation scanning electron microscopes Seawater VBNC Vibrio alginolyticus Vibrio alginolyticus - growth & development Vibrio alginolyticus - isolation & purification Vibrio alginolyticus - metabolism Vibrio alginolyticus - pathogenicity Water analysis
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Zusammenfassung:	The aim of this study was to investigate the viable but nonculturable (VBNC) state of the bacterium. Vibrio alginolyticus VIB283 was cultured in sterilized seawater microcosm at 4°C. Culturability of the cells in the microcosm was monitored by spread plate count (PC) on 2216E agar, PCs declined to undetectable levels (
ISSN:	0302-8933 1432-072X
DOI:	10.1007/s00203-007-0246-5