Characterization and application of monoclonal antibodies against channel catfish virus (CCV)

Characterization and application of monoclonal antibodies against channel catfish virus (CCV)

Three monoclonal antibodies (MAbs) (27E4, 17H2, 8B6) against channel catfish virus (CCV) were developed by immunizing Balb/C mice. Using indirect ELISA, these MAbs reacted only with CCV and not with three other fish viruses or nine fish cell lines. During western blotting analysis, MAb 27E4 recogniz...

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Veröffentlicht in:Archives of virology 2018-09, Vol.163 (9), p.2503-2506
Hauptverfasser: Jing, Hongli, Zhang, Min, Wu, Shaoqiang, Lin, Xiangmei, Gao, Longying, Wang, Na
Format: Artikel
Sprache:eng
Schlagworte:
Biomedical and Life Sciences
Biomedicine
Brief Report
Catfish
Cell lines
Enzyme-linked immunosorbent assay
Fish
Ictalurus punctatus
Infectious Diseases
Medical Microbiology
Monoclonal antibodies
Virology
Western blotting
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Beschreibung
Zusammenfassung:Three monoclonal antibodies (MAbs) (27E4, 17H2, 8B6) against channel catfish virus (CCV) were developed by immunizing Balb/C mice. Using indirect ELISA, these MAbs reacted only with CCV and not with three other fish viruses or nine fish cell lines. During western blotting analysis, MAb 27E4 recognized 170 kDa and 47 kDa proteins, while MAb 17H2 and MAb 8B6 recognized 47 kDa and 56 kDa proteins, respectively. Furthermore, a sandwich ELISA was developed for detection of CCV. The detection limit of the test was 10 5 TCID 50 /mL.
ISSN:0304-8608
1432-8798
DOI:10.1007/s00705-018-3884-9