Development of Sensitive Immunoassays for the Detection of the Glucuronide Conjugate of 3-Phenoxybenzyl Alcohol, a Putative Human Urinary Biomarker for Pyrethroid Exposure
Pyrethroids are widely used in agriculture as insecticides. This study describes a sensitive enzyme-linked immunosorbent assay for the detection of the glucuronide conjugate of 3-phenoxybenzyl alcohol, a putative pyrethroid metabolite that may be used as a biomarker of exposure to pyrethroids. Four...
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| Veröffentlicht in: | Journal of agricultural and food chemistry 2007-05, Vol.55 (10), p.3750-3757 |
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| container_title | Journal of agricultural and food chemistry |
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| creator | Kim, Hee-Joo Ahn, Ki Chang Ma, Seung Jin Gee, Shirley J. Hammock, Bruce D. |
| description | Pyrethroids are widely used in agriculture as insecticides. This study describes a sensitive enzyme-linked immunosorbent assay for the detection of the glucuronide conjugate of 3-phenoxybenzyl alcohol, a putative pyrethroid metabolite that may be used as a biomarker of exposure to pyrethroids. Four antisera were elicited against two different immunizing haptens. Antisera were characterized in combination with several coating haptens. The lowest IC50 value (0.5 ng/mL) was obtained with antiserum 1891 and 3-phenoxybenzoic acid−BSA conjugate as the coating antigen. Antiserum 1891 was highly selective for the target compound with an overall cross-reactivity of |
| doi_str_mv | 10.1021/jf063282g |
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This study describes a sensitive enzyme-linked immunosorbent assay for the detection of the glucuronide conjugate of 3-phenoxybenzyl alcohol, a putative pyrethroid metabolite that may be used as a biomarker of exposure to pyrethroids. Four antisera were elicited against two different immunizing haptens. Antisera were characterized in combination with several coating haptens. The lowest IC50 value (0.5 ng/mL) was obtained with antiserum 1891 and 3-phenoxybenzoic acid−BSA conjugate as the coating antigen. Antiserum 1891 was highly selective for the target compound with an overall cross-reactivity of <0.3% to structurally related compounds. The assay sensitivity was negligibly affected by pH 4−9. A 5-fold improvement in IC50 was observed using a 10-fold concentrated phosphate-fuffered saline as the assay buffer. Compared to assays conducted in normal phosphate-fuffered saline, the maximal absorbance was almost identical. A good correlation (r 2 = 0.99 and 0.97 for urine samples A and B, respectively) was observed between spiked levels and the levels detected by the immunoassay. Keywords: ELISA; pyrethroid; glucuronide conjugate; human exposure; hapten</description><identifier>ISSN: 0021-8561</identifier><identifier>EISSN: 1520-5118</identifier><identifier>DOI: 10.1021/jf063282g</identifier><identifier>PMID: 17455946</identifier><identifier>CODEN: JAFCAU</identifier><language>eng</language><publisher>Washington, DC: American Chemical Society</publisher><subject>3-phenoxybenzyl alcohol ; antiserum ; Benzyl Alcohols - urine ; Biological and medical sciences ; biomarkers ; Biomarkers - urine ; bovine serum albumin ; Environmental Exposure ; enzyme-linked immunosorbent assay ; Food industries ; Fundamental and applied biological sciences. Psychology ; General aspects ; glucuronide conjugate ; Glucuronides - urine ; Haptens ; Humans ; Hydrogen-Ion Concentration ; Immunoassay - methods ; metabolites ; Methods of analysis, processing and quality control, regulation, standards ; occupational exposure ; phenolic compounds ; Pyrethrins ; Reproducibility of Results ; Sensitivity and Specificity ; urine</subject><ispartof>Journal of agricultural and food chemistry, 2007-05, Vol.55 (10), p.3750-3757</ispartof><rights>Copyright © 2007 American Chemical Society</rights><rights>2007 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a502t-b30d684038b14662dd06996fb08b52cab324e257d7b61370619ebf396a5aa05b3</citedby><cites>FETCH-LOGICAL-a502t-b30d684038b14662dd06996fb08b52cab324e257d7b61370619ebf396a5aa05b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/jf063282g$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/jf063282g$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>309,310,314,776,780,785,786,2752,23909,23910,25118,27053,27901,27902,56713,56763</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=18748152$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17455946$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kim, Hee-Joo</creatorcontrib><creatorcontrib>Ahn, Ki Chang</creatorcontrib><creatorcontrib>Ma, Seung Jin</creatorcontrib><creatorcontrib>Gee, Shirley J.</creatorcontrib><creatorcontrib>Hammock, Bruce D.</creatorcontrib><title>Development of Sensitive Immunoassays for the Detection of the Glucuronide Conjugate of 3-Phenoxybenzyl Alcohol, a Putative Human Urinary Biomarker for Pyrethroid Exposure</title><title>Journal of agricultural and food chemistry</title><addtitle>J. Agric. Food Chem</addtitle><description>Pyrethroids are widely used in agriculture as insecticides. This study describes a sensitive enzyme-linked immunosorbent assay for the detection of the glucuronide conjugate of 3-phenoxybenzyl alcohol, a putative pyrethroid metabolite that may be used as a biomarker of exposure to pyrethroids. Four antisera were elicited against two different immunizing haptens. Antisera were characterized in combination with several coating haptens. The lowest IC50 value (0.5 ng/mL) was obtained with antiserum 1891 and 3-phenoxybenzoic acid−BSA conjugate as the coating antigen. Antiserum 1891 was highly selective for the target compound with an overall cross-reactivity of <0.3% to structurally related compounds. The assay sensitivity was negligibly affected by pH 4−9. A 5-fold improvement in IC50 was observed using a 10-fold concentrated phosphate-fuffered saline as the assay buffer. Compared to assays conducted in normal phosphate-fuffered saline, the maximal absorbance was almost identical. A good correlation (r 2 = 0.99 and 0.97 for urine samples A and B, respectively) was observed between spiked levels and the levels detected by the immunoassay. Keywords: ELISA; pyrethroid; glucuronide conjugate; human exposure; hapten</description><subject>3-phenoxybenzyl alcohol</subject><subject>antiserum</subject><subject>Benzyl Alcohols - urine</subject><subject>Biological and medical sciences</subject><subject>biomarkers</subject><subject>Biomarkers - urine</subject><subject>bovine serum albumin</subject><subject>Environmental Exposure</subject><subject>enzyme-linked immunosorbent assay</subject><subject>Food industries</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>General aspects</subject><subject>glucuronide conjugate</subject><subject>Glucuronides - urine</subject><subject>Haptens</subject><subject>Humans</subject><subject>Hydrogen-Ion Concentration</subject><subject>Immunoassay - methods</subject><subject>metabolites</subject><subject>Methods of analysis, processing and quality control, regulation, standards</subject><subject>occupational exposure</subject><subject>phenolic compounds</subject><subject>Pyrethrins</subject><subject>Reproducibility of Results</subject><subject>Sensitivity and Specificity</subject><subject>urine</subject><issn>0021-8561</issn><issn>1520-5118</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNptkVFv0zAUhSMEYmXwwB8AvwwJiYDtxHbyuHVjHaogUleJN8tJbtqUxC62MzX8Jf4k7lqtLzxYV_L5dI7uPVH0luDPBFPyZdNgntCMrp5FE8Iojhkh2fNogoMYZ4yTs-iVcxuMccYEfhmdEZEylqd8Ev29hgfozLYH7ZFp0AK0a337AOiu7wdtlHNqdKgxFvk1oGvwUPnW6D27_7jthmqwRrc1oKnRm2GlPOzFJC7WoM1uLEH_GTt02VVmbbpPSKFi8OoxYjb0SqOlbbWyI7pqTa_sL7CPacVowa-taWt0s9saN1h4Hb1oVOfgzXGeR8uvN_fTWTz_cXs3vZzHimHq4zLBNc9SnGQlSTmndY15nvOmxFnJaKXKhKZAmahFyUkiMCc5lE2Sc8WUwqxMzqMPB9-tNb8HcF72raug65QGMzhJcUo5znkAPx7AyhrnLDRya9uwwygJlvtm5FMzgX13NB3KHuoTeawiABdHQLlKdY1VumrdictEmoVyAxcfuNZ52D3p4XKSi0QweV8s5Ez8LNJv3-fyKvDvD3yjjFQrGzyXC4pJgrEQaXinZFU5uTGD1eG6_1nhH1LovNY</recordid><startdate>20070516</startdate><enddate>20070516</enddate><creator>Kim, Hee-Joo</creator><creator>Ahn, Ki Chang</creator><creator>Ma, Seung Jin</creator><creator>Gee, Shirley J.</creator><creator>Hammock, Bruce D.</creator><general>American Chemical Society</general><scope>FBQ</scope><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7U7</scope><scope>C1K</scope></search><sort><creationdate>20070516</creationdate><title>Development of Sensitive Immunoassays for the Detection of the Glucuronide Conjugate of 3-Phenoxybenzyl Alcohol, a Putative Human Urinary Biomarker for Pyrethroid Exposure</title><author>Kim, Hee-Joo ; Ahn, Ki Chang ; Ma, Seung Jin ; Gee, Shirley J. ; Hammock, Bruce D.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a502t-b30d684038b14662dd06996fb08b52cab324e257d7b61370619ebf396a5aa05b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>3-phenoxybenzyl alcohol</topic><topic>antiserum</topic><topic>Benzyl Alcohols - urine</topic><topic>Biological and medical sciences</topic><topic>biomarkers</topic><topic>Biomarkers - urine</topic><topic>bovine serum albumin</topic><topic>Environmental Exposure</topic><topic>enzyme-linked immunosorbent assay</topic><topic>Food industries</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>General aspects</topic><topic>glucuronide conjugate</topic><topic>Glucuronides - urine</topic><topic>Haptens</topic><topic>Humans</topic><topic>Hydrogen-Ion Concentration</topic><topic>Immunoassay - methods</topic><topic>metabolites</topic><topic>Methods of analysis, processing and quality control, regulation, standards</topic><topic>occupational exposure</topic><topic>phenolic compounds</topic><topic>Pyrethrins</topic><topic>Reproducibility of Results</topic><topic>Sensitivity and Specificity</topic><topic>urine</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kim, Hee-Joo</creatorcontrib><creatorcontrib>Ahn, Ki Chang</creatorcontrib><creatorcontrib>Ma, Seung Jin</creatorcontrib><creatorcontrib>Gee, Shirley J.</creatorcontrib><creatorcontrib>Hammock, Bruce D.</creatorcontrib><collection>AGRIS</collection><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Toxicology Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><jtitle>Journal of agricultural and food chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kim, Hee-Joo</au><au>Ahn, Ki Chang</au><au>Ma, Seung Jin</au><au>Gee, Shirley J.</au><au>Hammock, Bruce D.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Development of Sensitive Immunoassays for the Detection of the Glucuronide Conjugate of 3-Phenoxybenzyl Alcohol, a Putative Human Urinary Biomarker for Pyrethroid Exposure</atitle><jtitle>Journal of agricultural and food chemistry</jtitle><addtitle>J. Agric. Food Chem</addtitle><date>2007-05-16</date><risdate>2007</risdate><volume>55</volume><issue>10</issue><spage>3750</spage><epage>3757</epage><pages>3750-3757</pages><issn>0021-8561</issn><eissn>1520-5118</eissn><coden>JAFCAU</coden><abstract>Pyrethroids are widely used in agriculture as insecticides. This study describes a sensitive enzyme-linked immunosorbent assay for the detection of the glucuronide conjugate of 3-phenoxybenzyl alcohol, a putative pyrethroid metabolite that may be used as a biomarker of exposure to pyrethroids. Four antisera were elicited against two different immunizing haptens. Antisera were characterized in combination with several coating haptens. The lowest IC50 value (0.5 ng/mL) was obtained with antiserum 1891 and 3-phenoxybenzoic acid−BSA conjugate as the coating antigen. Antiserum 1891 was highly selective for the target compound with an overall cross-reactivity of <0.3% to structurally related compounds. The assay sensitivity was negligibly affected by pH 4−9. A 5-fold improvement in IC50 was observed using a 10-fold concentrated phosphate-fuffered saline as the assay buffer. Compared to assays conducted in normal phosphate-fuffered saline, the maximal absorbance was almost identical. A good correlation (r 2 = 0.99 and 0.97 for urine samples A and B, respectively) was observed between spiked levels and the levels detected by the immunoassay. Keywords: ELISA; pyrethroid; glucuronide conjugate; human exposure; hapten</abstract><cop>Washington, DC</cop><pub>American Chemical Society</pub><pmid>17455946</pmid><doi>10.1021/jf063282g</doi><tpages>8</tpages></addata></record> |
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| subjects | 3-phenoxybenzyl alcohol antiserum Benzyl Alcohols - urine Biological and medical sciences biomarkers Biomarkers - urine bovine serum albumin Environmental Exposure enzyme-linked immunosorbent assay Food industries Fundamental and applied biological sciences. Psychology General aspects glucuronide conjugate Glucuronides - urine Haptens Humans Hydrogen-Ion Concentration Immunoassay - methods metabolites Methods of analysis, processing and quality control, regulation, standards occupational exposure phenolic compounds Pyrethrins Reproducibility of Results Sensitivity and Specificity urine |
| title | Development of Sensitive Immunoassays for the Detection of the Glucuronide Conjugate of 3-Phenoxybenzyl Alcohol, a Putative Human Urinary Biomarker for Pyrethroid Exposure |
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