Development of Sensitive Immunoassays for the Detection of the Glucuronide Conjugate of 3-Phenoxybenzyl Alcohol, a Putative Human Urinary Biomarker for Pyrethroid Exposure

Development of Sensitive Immunoassays for the Detection of the Glucuronide Conjugate of 3-Phenoxybenzyl Alcohol, a Putative Human Urinary Biomarker for Pyrethroid Exposure

Pyrethroids are widely used in agriculture as insecticides. This study describes a sensitive enzyme-linked immunosorbent assay for the detection of the glucuronide conjugate of 3-phenoxybenzyl alcohol, a putative pyrethroid metabolite that may be used as a biomarker of exposure to pyrethroids. Four...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Journal of agricultural and food chemistry 2007-05, Vol.55 (10), p.3750-3757
Hauptverfasser: Kim, Hee-Joo, Ahn, Ki Chang, Ma, Seung Jin, Gee, Shirley J., Hammock, Bruce D.
Format: Artikel
Sprache:eng
Schlagworte:
3-phenoxybenzyl alcohol
antiserum
Benzyl Alcohols - urine
Biological and medical sciences
biomarkers
Biomarkers - urine
bovine serum albumin
Environmental Exposure
enzyme-linked immunosorbent assay
Food industries
Fundamental and applied biological sciences. Psychology
General aspects
glucuronide conjugate
Glucuronides - urine
Haptens
Humans
Hydrogen-Ion Concentration
Immunoassay - methods
metabolites
Methods of analysis, processing and quality control, regulation, standards
occupational exposure
phenolic compounds
Pyrethrins
Reproducibility of Results
Sensitivity and Specificity
urine
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:Pyrethroids are widely used in agriculture as insecticides. This study describes a sensitive enzyme-linked immunosorbent assay for the detection of the glucuronide conjugate of 3-phenoxybenzyl alcohol, a putative pyrethroid metabolite that may be used as a biomarker of exposure to pyrethroids. Four antisera were elicited against two different immunizing haptens. Antisera were characterized in combination with several coating haptens. The lowest IC50 value (0.5 ng/mL) was obtained with antiserum 1891 and 3-phenoxybenzoic acid−BSA conjugate as the coating antigen. Antiserum 1891 was highly selective for the target compound with an overall cross-reactivity of
ISSN:0021-8561
1520-5118
DOI:10.1021/jf063282g