Development of an opsonophagocytic killing assay for group a streptococcus

•OPKA developed for a number of clinically relevant Group A Streptococcus.•Homologous M-protein antisera shown to kill GAS emm-types 1, 6 and 12.•Killing inhibited by homologous but not heterologous antigens proving specificity.•GAS OPKA is precise; with intra and inter-assay coefficients of variati...

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Veröffentlicht in:	Vaccine 2018-06, Vol.36 (26), p.3756-3763
Hauptverfasser:	Jones, Scott, Moreland, Nicole J., Zancolli, Marta, Raynes, Jeremy, Loh, Jacelyn M.S., Smeesters, Pierre R., Sriskandan, Shiranee, Carapetis, Jonathan R., Fraser, John D., Goldblatt, David
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Antibodies, Bacterial - blood Antigens Antisera Assaying Baby rabbit complement Cell Line Complement Complement System Proteins - immunology Dimethylformamide Functional assay GAS vaccines Gram-positive bacteria HL-60 cells Homology Humans Immunoassay - methods Immunoassay - standards Immunoglobulins Leukemia Leukocytes (neutrophilic) Linearity Microbial Viability Neutrophils - immunology Opsonin Proteins - blood Opsonophagocytic killing assay Phagocytosis Promyeloid leukemia Proteins Rabbits Sensitivity and Specificity Streptococcal Infections - immunology Streptococcus Streptococcus infections Streptococcus pyogenes Streptococcus pyogenes - immunology Streptococcus pyogenes - physiology Vaccine development Vaccines
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Beschreibung
Zusammenfassung:	•OPKA developed for a number of clinically relevant Group A Streptococcus.•Homologous M-protein antisera shown to kill GAS emm-types 1, 6 and 12.•Killing inhibited by homologous but not heterologous antigens proving specificity.•GAS OPKA is precise; with intra and inter-assay coefficients of variation
ISSN:	0264-410X 1873-2518
DOI:	10.1016/j.vaccine.2018.05.056