Callus Induction and Organogenesis from Explants of Aconitum heterophyllum- Medicinal Plant

Callus Induction and Organogenesis from Explants of Aconitum heterophyllum- Medicinal Plant

A protocol has been developed for in vitro shoot proliferation from callus cultures of Aconitum heterophyllum Wall. Callus initiation occurs from nodal segments on MS media fortified with NAA (0.5 mg L super(-1)) and BAP (0.25 mg L super(-1)). Callus was transferred on Msmediasupplemented with BAP (...

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Veröffentlicht in:Biotechnology (Faisalābād, Pakistan) Pakistan), 2006, Vol.5 (3), p.287-291
Hauptverfasser: Jabeen, N, Shawl, A S, Dar, G H, Jan, A, Sultan, P
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Aconitum
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container_start_page 287
container_title Biotechnology (Faisalābād, Pakistan)
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creator Jabeen, N
Shawl, A S
Dar, G H
Jan, A
Sultan, P
description A protocol has been developed for in vitro shoot proliferation from callus cultures of Aconitum heterophyllum Wall. Callus initiation occurs from nodal segments on MS media fortified with NAA (0.5 mg L super(-1)) and BAP (0.25 mg L super(-1)). Callus was transferred on Msmediasupplemented with BAP (0.25 mg L super(-1)) for shoot proliferation. The best response for shoot proliferation was obtained on MS media+ NAA (0.25 mg L super(-1)) + BAP (0.5 mg L super(-1)). The well-developed micro shoots were transferred to root induction media containing MS basalmedia + IAA (1.0 mg L super(-1)). The rooted plantlets were finally transferred to green house for hardening and field transfer.
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title Callus Induction and Organogenesis from Explants of Aconitum heterophyllum- Medicinal Plant
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