Callus Induction and Organogenesis from Explants of Aconitum heterophyllum- Medicinal Plant

A protocol has been developed for in vitro shoot proliferation from callus cultures of Aconitum heterophyllum Wall. Callus initiation occurs from nodal segments on MS media fortified with NAA (0.5 mg L super(-1)) and BAP (0.25 mg L super(-1)). Callus was transferred on Msmediasupplemented with BAP (0.25 mg L super(-1)) for shoot proliferation. The best response for shoot proliferation was obtained on MS media+ NAA (0.25 mg L super(-1)) + BAP (0.5 mg L super(-1)). The well-developed micro shoots were transferred to root induction media containing MS basalmedia + IAA (1.0 mg L super(-1)). The rooted plantlets were finally transferred to green house for hardening and field transfer.