Kinetics of Canine Antibody Response to Saliva of the Sand Fly Lutzomyia longipalpis
Zoonotic visceral leishmaniosis (VL) caused by Leishmania infantum is transmitted from dogs to humans by sand flies and Lutzomyia longipalpis is a major vector of this disease. We studied the antibody response in dogs experimentally exposed to L. longipalpis females to characterize sand fly salivary...
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Veröffentlicht in: | Vector borne and zoonotic diseases (Larchmont, N.Y.) N.Y.), 2008-08, Vol.8 (4), p.443-450 |
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Sprache: | eng |
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Zusammenfassung: | Zoonotic visceral leishmaniosis (VL) caused by
Leishmania infantum
is transmitted from dogs to humans by sand flies and
Lutzomyia longipalpis
is a major vector of this disease. We studied the antibody response in dogs experimentally exposed to
L. longipalpis
females to characterize sand fly salivary antigens recognized by canine sera and to find out whether the level of specific anti-saliva antibodies reflects the intensity of exposure. Sera from repeatedly bitten dogs revealed up to six salivary protein bands with approximate molecular weight of 66, 55, 45, 37-39, 34, and 25 kDa in
L. longipalpis
salivary gland lysate. Anti-saliva immunoglobin (Ig) G and its subclasses were found to be useful markers of exposure to sand flies. Specific IgG, IgG1, and IgG2 were related to numbers of bloodfed
L. longipalpis
females, and increased antibody levels were detectable throughout the study, i.e. more than 6 months after the last exposure. In contrast, specific IgE response developed in some dogs only, and no correlation was observed between its level and the intensity of exposure. Screening of dog sera for specific IgG against salivary antigens of the vector is suggested as a useful epidemiological tool in VL foci. Monitoring canine antibody response to sand fly saliva also allows evaluation of the effectiveness of anti-vector campaigns. |
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ISSN: | 1530-3667 1557-7759 |
DOI: | 10.1089/vbz.2007.0214 |