Accuracy of Burkholderia pseudomallei Identification Using the API 20NE System and a Latex Agglutination Test

In an evaluation of the API 20NE for the identification of Burkholderia spp., 792/800 (99%) Burkholderia pseudomallei and 17/19 (89%) B. cepacia isolates were correctly identified but 10 B. mallei and 98 B. thailandensis isolates were not correctly identified. A latex agglutination test was positive...

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Veröffentlicht in:	Journal of Clinical Microbiology 2007-11, Vol.45 (11), p.3774-3776
Hauptverfasser:	Amornchai, Premjit, Chierakul, Wirongrong, Wuthiekanun, Vanaporn, Mahakhunkijcharoen, Yuvadee, Phetsouvanh, Rattanaphone, Currie, Bart J, Newton, Paul N, van Vinh Chau, Nguyen, Wongratanacheewin, Surasakdi, Day, Nicholas P.J, Peacock, Sharon J
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Bacteriology Biological and medical sciences Burkholderia pseudomallei Burkholderia pseudomallei - isolation & purification Diagnostic Errors Fundamental and applied biological sciences. Psychology Humans Latex Fixation Tests - methods Microbiology Miscellaneous Reagent Kits, Diagnostic
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Zusammenfassung:	In an evaluation of the API 20NE for the identification of Burkholderia spp., 792/800 (99%) Burkholderia pseudomallei and 17/19 (89%) B. cepacia isolates were correctly identified but 10 B. mallei and 98 B. thailandensis isolates were not correctly identified. A latex agglutination test was positive for 796/800 (99.5%) B. pseudomallei isolates and negative for 120 other oxidase-positive gram-negative bacilli.
ISSN:	0095-1137 1098-660X 1098-5530
DOI:	10.1128/JCM.00935-07