New biochemical methods for production of N-benzyloxycarbonyl-d,l-aminoadipic- delta -semialdehyde and N-benzyloxycarbonyl-d,l-aminoadipic acid

New biochemical methods for the production of N alpha -benzyloxycarbonyl-l-aminoadipate delta -semialdehyde (N alpha -Z-l-AASA), N alpha -Z-d-AASA, N alpha -Z-l-aminoadipic acid (N alpha -Z-l-AAA), N alpha -Z-d-AAA were developed using cells of Rhodococcus sp. AIU Z-35-1. When the cells harvested after 1 day of cultivation were incubated with 100mM N alpha -Z-l-lysine at pH 5.0 for 1 day at 30°C or at pH 7.0 for 2 days at 10°C, more than 95mM N alpha -Z-l-AASA was produced. Using the cells harvested after 3 days of cultivation, N alpha -Z-l-AAA was efficiently produced by incubating at pH 7.0 for 4 days at 30°C. Similar conversion yields of N alpha -Z-d-AASA and N alpha -Z-d-AAA were also obtained under the same conditions. Thus, the microbial methods proposed here were superior to the chemical and other biochemical methods in simplicity, reaction rate, and yield.