Facile construction of unmarked deletion mutants in Burkholderia pseudomallei using sacB counter-selection in sucrose-resistant and sucrose-sensitive isolates

Burkholderia pseudomallei is the causative agent of melioidosis, a potentially fatal disease endemic or emerging world-wide. Here we report unmarked allele-replacement mutagenesis using efficient sacB counter-selection. Despite being genotypically sacB +, most commonly used B. pseudomallei strains a...

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Veröffentlicht in:	Journal of microbiological methods 2009-03, Vol.76 (3), p.320-323
Hauptverfasser:	Logue, Carie-Anne, Peak, Ian R.A., Beacham, Ifor R.
Format:	Artikel
Sprache:	eng
Schlagworte:	Alleles Bacteriology Biological and medical sciences Burkholderia pseudomallei Burkholderia pseudomallei - genetics Burkholderia pseudomallei - metabolism Counter-selection DNA, Bacterial - genetics Fundamental and applied biological sciences. Psychology Gene expression Gene Expression Regulation, Bacterial Genes, Bacterial Genetics Microbiology Molecular and cellular biology Molecular genetics Mutagenesis, Site-Directed - methods sacB Sequence Deletion Sucrose - metabolism Unmarked deletions
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Beschreibung
Zusammenfassung:	Burkholderia pseudomallei is the causative agent of melioidosis, a potentially fatal disease endemic or emerging world-wide. Here we report unmarked allele-replacement mutagenesis using efficient sacB counter-selection. Despite being genotypically sacB +, most commonly used B. pseudomallei strains are sucrose-resistant and efficient sacB counter-selection is demonstrated in both resistant and sensitive strains.
ISSN:	0167-7012 1872-8359
DOI:	10.1016/j.mimet.2008.12.007