Real-Time Characterization of Ribozymes by Fluorecence Resonance Energy Transfer (FRET)

Real-Time Characterization of Ribozymes by Fluorecence Resonance Energy Transfer (FRET)

More rapid than with conventional methods is the analysis of ribozyme kinetics upon use of FRET substrates. In these substrates a fluorophore and a fluorescence‐quenching molecule lie in close spatial proximity. The intramolecular fluorescence quenching is neutralized upon cleavage, and the fluoresc...

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Veröffentlicht in:Angewandte Chemie International Edition 1999-05, Vol.38 (9), p.1300-1303
Hauptverfasser: Jenne, Andreas, Gmelin, Walter, Raffler, Nikolai, Famulok, Michael
Format: Artikel
Sprache:eng
Schlagworte:
Fluorescence spectroscopy
Hammerhead ribozyme
Ribozymes
Screening methods
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Beschreibung
Zusammenfassung:More rapid than with conventional methods is the analysis of ribozyme kinetics upon use of FRET substrates. In these substrates a fluorophore and a fluorescence‐quenching molecule lie in close spatial proximity. The intramolecular fluorescence quenching is neutralized upon cleavage, and the fluorescence intensity is a measure of the ribozyme activity. By automation and computer assistance the activity of ribozymes can be monitored under high‐throughput conditions.
ISSN:1433-7851
1521-3773
DOI:10.1002/(SICI)1521-3773(19990503)38:9<1300::AID-ANIE1300>3.0.CO;2-Q