A High-Throughput-Screening Method for the Identification of Active and Enantioselective Hydrolases

A High-Throughput-Screening Method for the Identification of Active and Enantioselective Hydrolases

A rapid and reliable test for the determination of hydrolase activity and enantioselectivity comprises the conversion of acetic acid released from acetates to NADH by using a commercially available enzymatic test‐kit (see scheme). The NADH is spectrophotometrically quantified in a microtiter plate f...

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Veröffentlicht in:Angewandte Chemie International Edition 2001-11, Vol.40 (22), p.4201-4204
Hauptverfasser: Baumann, Markus, Stürmer, Rainer, Bornscheuer, Uwe T.
Format: Artikel
Sprache:eng
Schlagworte:
biotransformations
enantioselectivity
enzyme catalysis
high-throughput screening
molecular evolution
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Beschreibung
Zusammenfassung:A rapid and reliable test for the determination of hydrolase activity and enantioselectivity comprises the conversion of acetic acid released from acetates to NADH by using a commercially available enzymatic test‐kit (see scheme). The NADH is spectrophotometrically quantified in a microtiter plate format.
ISSN:1433-7851
1521-3773
DOI:10.1002/1521-3773(20011119)40:22<4201::AID-ANIE4201>3.0.CO;2-V