miR-375 prevents nasal mucosa cells from apoptosis and ameliorates allergic rhinitis via inhibiting JAK2/STAT3 pathway

[Display omitted] •A new critical role of miR-375 in allergic rhinitis is proposed.•The mechanisms of miR-375 in allergic rhinitis were further studied.•miR-375/JAK2/STAT3 regulatory axis was firstly identified in nasal mucosa cells.•The novel mechanism provides new potential targets for allergic rhinitis treatment. This study aims to explore the roles, and related mechanisms of miR-375 in nasal mucosa cells apoptosis and allergic rhinitis. Here, miR-375 was found to be decreased in the epithelia of nasal mucosa of allergic rhinitis mice and TNF-α-stimulated nasal mucosa cells, while JAK2 expression exhibited an opposite effect. Mechanistically, miR-375/JAK2 regulatory axis was identified in nasal mucosa cells via luciferase reporter, qRT-PCR, western blot and RNA immune co-precipitation (RIP) assays. Pre- or post-injection of miR-375 agomir following ovalbumin (OVA) treatment attenuated OVA-induced allergic rhinitis, characterized as the downregulation of IL-6 and TNF-α secretion, and upregulation of IL-10 secretion, these effects were attenuated by infection with JAK2 adenovirus through nasal cavity inhalation. Additionally, overexpression of miR-375 reversed TNF-α-mediated nasal mucosa cells apoptosis, which was attenuated by JAK2 overexpression. Therefore, our results indicate that miR-375 prevents nasal mucosa cells from apoptosis and ameliorates allergic rhinitis via inhibiting JAK2/STAT3 pathway.