Recombinant human granulocyte-macrophage colony-stimulating factor: effect of glycosylation on pharmacokinetic parameters
The pharmacokinetic behaviour of the non-glycosylated, bacterially-derived recombinant human granulocyte-macrophage colony-stimulating factor (rhGM-CSF) and the glycosylated mammalian product was studied after intra and extra vascular administration of a single dose in rodents. Each route of adminis...
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Veröffentlicht in: | Electronic Journal of Biotechnology 2007-04, Vol.10 (2) |
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Hauptverfasser: | , , , |
Format: | Artikel |
Sprache: | eng |
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Online-Zugang: | Volltext |
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Zusammenfassung: | The pharmacokinetic behaviour of the non-glycosylated,
bacterially-derived recombinant human granulocyte-macrophage
colony-stimulating factor (rhGM-CSF) and the glycosylated mammalian
product was studied after intra and extra vascular administration of a
single dose in rodents. Each route of administration gave a different
rhGM-CSF concentration-time profile. After extra vascular
administration of equivalent doses, a higher peak concentration and
faster elimination were observed in the group treated with the E.
coli-derived cytokine. The faster elimination resulted in a return to
pre-treatment plasma levels after 12 hrs, versus 48 hrs following the
administration of glycosylated rhGM-CSF. After intravascular
administration, clearance of rhGM-CSF was significantly decreased by
the presence of carbohydrates. Non-significant differences in the
terminal phase of the biphasic kinetics were found, but the
distribution phase was significantly longer for the glycosylated form. |
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ISSN: | 0717-3458 0717-3458 |
DOI: | 10.2225/vol10-issue2-fulltext-5 |