Rapid micropropagation of three elite Sugarcane (Saccharum officinarum L.) varieties by shoot tip culture

Studies were carried out for rapid micropropagation of three sugarcane varieties i.e., HSF-240, CP-77-400 and CPF-237. Shoot tip was used as explant source. Shoot initiation from explant of all three varieties was achieved at 1 mg/l Kin and 0.1 mg/l GA sub(3). For rapid multiplication the regenerated shoots were transferred on liquid Murashige and Skoog medium containing 2% sucrose, supplemented with BAP in combinations with GA sub(3). Optimum multiplication was observed at 1 mg/l BAP in combination with 0.1 mg/l GA sub(3) for variety HSF-240. Best response of multiplication for variety CP-77-400 was observed at 0.5 mg/l BAP with 0.1 mg/l GA sub(3). Variety CPF-237 was multiplied at 1.0 mg/l BAP with 0.5 mg/l GA sub(3). Rooting response was observed on half strength liquid MS medium with 6% sucrose containing different concentrations of IBA and NAA. The sugarcane plantlets were acclimatized in greenhouse.