Evaluation of the AdvanDx VRE EVIGENE Assay for Detection of vanA in Vancomycin-Resistant Staphylococcus aureus

AdvanDx VRE EVIGENE, a commercial vanA/vanB DNA hybridization assay to identify vancomycin-resistant enterococci (VRE), was evaluated for the detection of vanA in Staphylococcus aureus. Performance was assessed using S. aureus, VRE, and vancomycin-intermediate and -susceptible isolates. The assay de...

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Veröffentlicht in:	Journal of Clinical Microbiology 2007-05, Vol.45 (5), p.1611-1613
Hauptverfasser:	Fosheim, G.E, Carey, R.B, Limbago, B.M
Format:	Artikel
Sprache:	eng
Schlagworte:	Bacterial Proteins - analysis Bacterial Proteins - genetics Bacteriology Biological and medical sciences Carbon-Oxygen Ligases - analysis Carbon-Oxygen Ligases - genetics DNA, Bacterial Fundamental and applied biological sciences. Psychology Infectious diseases Medical sciences Microbial Sensitivity Tests Microbiology Miscellaneous Nucleic Acid Hybridization - methods Sensitivity and Specificity Staphylococcus aureus Staphylococcus aureus - drug effects Staphylococcus aureus - genetics Vancomycin Resistance
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Beschreibung
Zusammenfassung:	AdvanDx VRE EVIGENE, a commercial vanA/vanB DNA hybridization assay to identify vancomycin-resistant enterococci (VRE), was evaluated for the detection of vanA in Staphylococcus aureus. Performance was assessed using S. aureus, VRE, and vancomycin-intermediate and -susceptible isolates. The assay demonstrated 100% sensitivity and specificity when analyzed visually and by optical density.
ISSN:	0095-1137 1098-660X 1098-5530
DOI:	10.1128/JCM.02556-06