Total choline quantification measured by 1H MR spectroscopy as early predictor of response after neoadjuvant treatment for locally advanced breast cancer: The impact of immunohistochemical status

Background Validation of new biomarkers is essential for the early evaluation of neoadjuvant treatments. Purpose To determine whether measurements of total choline (tCho) by 1H spectroscopy could predict morphological or pathological complete response (pCR) of neoadjuvant treatment and whether breast cancer subgroups are related to prediction accuracy. Study Type Prospective, nonrandomized, monocentric, diagnostic study. Population Sixty patients were initially included with 39 women participating in the final cohort. Field Strength/Sequence A 1.5T scanner was used for acquisition and MRS was performed using the syngo GRACE sequence. Assessment MRS and MRI examinations were performed at baseline (TP1), 24–72 hours after first chemotherapy (TP2), after the end of anthracycline treatment (TP3), and MRI only after the end of taxane treatment (TP4). Early (EMR) and late (LMR) morphological response were defined as %ΔDmax13 or %ΔDmax14, respectively. Responders were patients with %ΔDmax >30. Pathological complete response (pCR) patients achieved a residual cancer burden score of 0. Statistical Tests T‐test, receiver operating characteristic (ROC) curves, multiple regression, logistic regression, one‐way analysis of variance (ANOVA) analysis were used for the analysis. Results At TP1 there was a significant difference between response groups for tCho1 concerning EMR prediction (P = 0.05) and pCR (P