Allergenicity assessment of transgenic mustard (Brassica juncea) expressing bacterial codA gene

Background: Assessing the allergenicity and toxicity of genetically modified (GM) crops is essential before they become a regular part of our food supply. The present study aimed to assess the allergenicity of Brassica juncea (mustard) expressing choline oxidase (codA) gene from Arthrobacter globiformis that provides resistance against abiotic stresses. Methods: SDAP, Farrp, and Swiss‐Prot databases were used to study allergenicity of choline oxidase. Digestibility of choline oxidase was assessed in simulated gastric fluid (SGF). Specific immunoglobulin E (IgE) reactivity of native and GM mustard was compared by using enzyme‐linked immunosorbent assay (ELISA) and skin tests in respiratory‐allergic patients. Allergenicity of GM and native mustard proteins was compared in Balb/c mice. Results: Choline oxidase showed no significant homology with allergenic proteins in SDAP and Farrp databases. Cross‐reactive epitope search showed a stretch similar to Hev b 6 having some antigenic properties. Purified choline oxidase showed complete degradation with SGF. Skin prick test of native and GM mustard extract on respiratory allergic patients showed significant correlation (P