Genetic Fingerprint of Some KSA Date Palm Cultivars Using Modern Biotechnological Techniques

In the present research six cultivars of date palm (Sukkari, Sifri, Sullag, Khalas, Makfazi and Maktoum) growing in the west region of KSA were investigated for genetic diversity using RAPD technique, total protein, protein patterns and amino acids analysis. Total genomic DNA extracted from the young leaves of six different cultivars. Ten decamer oligonucleotides were used as primers under optimized PCR conditions. Polymorphisms were recorded by noting the presence or absence of an amplification product from the total genomic DNA. A total of 303 DNA fragment were produced from all experiments with an average of 5.05 bands per primer and cultivar. The total number of monomorphic fragments was 222/330. While, the total number of polymorphic fragment was 81/303 fragments. In addition to amino acids analysis and protein banding were determined.