Effect of yeast supplementation on the in vitro fermentation of high-temperature dried lucerne incubated with an equine faecal inoculum

An in vitro gas production (GP) experiment, using equine faeces as the source of inoculum, was conducted to assess the effect of a yeast preparation ( Saccharomyces cerevisiae) on the fermentation of high-temperature dried lucerne. The experiment was a factorial design consisting of six levels of yeast supplementation of high-temperature dried lucerne; 0 g yeast g −1 ( L 0—Control), 2 g yeast g −1 ( L 1), 4 g yeast g −1 ( L 2), 8 g yeast g −1 ( L 3), 16 g yeast g −1 ( L 4) and 40 g yeast g −1 ( L 5) herbage added at two time points; time 0 (inoculation) and at hour 14 of the incubation to 4 replicate bottles (5 × 2 × 4). Total GP increased significantly (P