ANTIMICROBIAL AND ANTIBIOFILM EFFECTS OF EXTRACTS FROM TRAPA NATANS L., EVALUATION OF TOTAL PHENOLIC AND FLAVONOID CONTENTS AND GC-MS ANALYSIS

Research conducted in this study shows the applied in vitro antimicrobial and antibiofilm activity of the four extracts isolated from Trapa natans L. leaves. In this study, different methods were used (microdilution, tissue culture plate, different colorimetric methods, GC-FID and GC-MS analysis). W...

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Veröffentlicht in:	Acta Poloniae pharmaceutica 2016-11, Vol.73 (6), p.1565-1574
Hauptverfasser:	Radojevic, Ivana D, Vasic, Sava M, Dekic, Milan S, Radulovic, Niko S, Delic, Gorica T, Durdevic, Jelena S, Comic, Ljiljana R
Format:	Artikel
Sprache:	eng
Schlagworte:	Anti-Bacterial Agents - isolation & purification Anti-Bacterial Agents - pharmacology Biofilms - drug effects Flavonoids - isolation & purification Flavonoids - pharmacology Gas Chromatography-Mass Spectrometry - methods Lythraceae - chemistry Microbial Sensitivity Tests Phenols - chemistry Phenols - isolation & purification Plant Extracts - chemistry Plant Extracts - pharmacology Plant Leaves
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Zusammenfassung:	Research conducted in this study shows the applied in vitro antimicrobial and antibiofilm activity of the four extracts isolated from Trapa natans L. leaves. In this study, different methods were used (microdilution, tissue culture plate, different colorimetric methods, GC-FID and GC-MS analysis). While the water extract didn't show antibacterial activity, the acetone extract showed the strongest one. The same activity in the case of Pseudomonas aeruginosa (MIC was 313 μg/mL) was better than the activity of controls and it matched with antibiofilm activity. The effect of extracts was better on G+ bacteria (MICs were
ISSN:	0001-6837