Effect of an experimental mouth rinse containing NaF and TiF4 on tooth erosion and abrasion in situ

This study compared the effect of an experimental NaF/TiF4 mouth rinse with a commercial tin/F mouth rinse on the prevention of tooth wear in situ. Fifteen subjects took part in this crossover and double-blind study, in which they wore a palatal appliance with 8 bovine teeth samples (4 enamel and 4 root dentine) in each of 3 phases (5 days each). Half of the samples were subjected to erosive challenges, and the other half to erosive plus abrasive challenges. The phases corresponded to the use of 1) Experimental solution containing NaF/TiF4 (189 ppm Ti+4, 500 ppm F−, pH 4.4); 2) commercial solution containing SnCl2/NaF/AmF (800 ppm Sn+2, 500 ppm F−, pH 4.5, Elmex®/GABA, positive control); 3) distilled water (negative control). Erosive challenges were performed using 0.1% citric acid (pH 2.5) for 90 s 4 times per day. The abrasion was done using a toothbrush and slurry of fluoride toothpaste, for 15 s 2 times per day. Thereafter, the subjects rinsed with the tested mouth rinse for 60s. Tooth wear was measured using contact profilometry (μm) and submitted to a two-way RM ANOVA/Bonferroni test (p