REVERSE PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY METHOD FOR DETERMINATION OF 5-FLUOROURACIL IN RABBIT PLASMA

A simple, efficient, accurate and selective HPLC method has been developed and validated successfully for the estimation of 5-fluorouracil in rabbit plasma. The drug was eluted by using Supelco C18 column (1.5 cm x 4.6 mm, 5 μm) with a mobile phase consisting of methanol and water (20: 80, v/v) by a...

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Veröffentlicht in:	Acta Poloniae pharmaceutica 2017-03, Vol.74 (2), p.379-383
Hauptverfasser:	Nasir, Bushra, Muhammad Ranjha, Nazar, Hanif, Muhammad, Abbas, Ghulam
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Antimetabolites, Antineoplastic - blood Antimetabolites, Antineoplastic - pharmacokinetics Buffers Calibration Chromatography, High Pressure Liquid - standards Chromatography, Reverse-Phase - standards Drug Monitoring - methods Fluorouracil - blood Fluorouracil - pharmacokinetics Hydrogen-Ion Concentration Limit of Detection Linear Models Methanol - chemistry Perchlorates - chemistry Rabbits Reference Standards Reproducibility of Results Solvents - chemistry Water - chemistry
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Beschreibung
Zusammenfassung:	A simple, efficient, accurate and selective HPLC method has been developed and validated successfully for the estimation of 5-fluorouracil in rabbit plasma. The drug was eluted by using Supelco C18 column (1.5 cm x 4.6 mm, 5 μm) with a mobile phase consisting of methanol and water (20: 80, v/v) by adjusting pH at 3.2, using perchloric acid solution. The retention time was found to be 4.107 with a flow rate of I mL/min. Multiple validation parameters evaluated with high accuracy indicating that the current method sufficiently qualifies the recommended criteria. Regression value obtained from linearity curve was R² = 0.999 and percentage recovery obtained was ranged from 96.6 to 102.5%. A fine response in short run time with perfect resolution made the method highly suitable for pharmacokinetic studies.
ISSN:	0001-6837