Thoughts and Progress: Pyruvate Improves Neutrophilic Nitric Oxide Generation in Peritoneal Dialysis Solutions

To investigate the effects of pyruvate (Pyr)-based peritoneal dialysis solutions (P-PDS) on neutrophilic nitric oxide (NO) generation, we incubated human peripheral neutrophils in dL-lactate (Lac, 40 mM)-based PDS and equimolar P-PDS, and Hanks' balanced salt solution at various pH and high glu...

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Veröffentlicht in:Artificial organs 2005-12, Vol.29 (12), p.976-980
Hauptverfasser: Wu, Yi-Tai, Wu, Zhao-Long, Jiang, Xiao-Feng, Li, Su, Zhou, Fang-Qiang
Format: Artikel
Sprache:eng
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Zusammenfassung:To investigate the effects of pyruvate (Pyr)-based peritoneal dialysis solutions (P-PDS) on neutrophilic nitric oxide (NO) generation, we incubated human peripheral neutrophils in dL-lactate (Lac, 40 mM)-based PDS and equimolar P-PDS, and Hanks' balanced salt solution at various pH and high glucose (HG) levels, respectively. The production of NO in various test solutions was determined based on the Griess reaction. Acidic pH, high Lac, and HG induced an acute and substantial inhibition of neutrophilic NO, whereas Pyr in PDS significantly improved the NO generation in both acidic pH and physiological pH, and also in HG conditions. The Pyr protection may be associated with the improvement of glucose metabolic pathways in addition to its alkalization.
ISSN:0160-564X
1525-1594
DOI:10.1111/j.1525-1594.2005.00167.x