EFFECT OF MAXIMAL EXERCISE ON NADPH OXIDASE ACTIVITY OF NEUTROPHILS AND RELATION TO SUPEROXIDE-GENERATING ACTIVITY BY CELL-SYSTEM IN HUMAN PERIPHERAL BLOOD
The purpose of this study was to examine reduced nicotinamide adenine dinucleotide phosphate (NADPH) oxidase activity, - a generated superoxide - of neutrophils in human peripheral blood after maximal exercise. ten healthy male college students (20.2 +/- 0.4 yr, 170.5 +/- 1.3 cm, 62.8 +/- 1.9 kg) pa...
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Veröffentlicht in: | Tairyoku kagaku. Japanese journal of physical fitness and sports medicine 2009, Vol.58 (2), p.255-264 |
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Hauptverfasser: | , , , |
Format: | Artikel |
Sprache: | eng |
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Zusammenfassung: | The purpose of this study was to examine reduced nicotinamide adenine dinucleotide phosphate (NADPH) oxidase activity, - a generated superoxide - of neutrophils in human peripheral blood after maximal exercise. ten healthy male college students (20.2 +/- 0.4 yr, 170.5 +/- 1.3 cm, 62.8 +/- 1.9 kg) participated after giving written informed consent. They performed an incremental exercise to volitional exhaustion using a bicycle ergometer. Peripheral blood was collected before exercise (Pre), just after exercise (Post) and 1-hour after exercise (Post-1h). Phorbol 12-myristate 13-acetate (PMA)-stimulated and opsonaized zymosan (OZ)-stimulated superoxide-generating activity of neutrophils was measured by the cytochrome c reduction assay. NADPH oxidase activity was measured by a cell-free system. NADPH oxidase activity significantly decreased in Post-1h compared with Pre and Post. A similar tendency was seen in PMA-stimulated activity, but not in OZ- stimulated activity. A strong positive relationship between NADPH oxidase activity and PMA-stimulated activity was found in Pre and this relationship attenuated after exercise. NADPH oxidase activity was not related to OZ- stimulated activity at any time points. We concluded that NADPH oxidase activity decreased after exhaustive maximal exercise in human peripheral neutrophils, and suggest that PMA-stimulated activity, relatively - speaking, reflects NADPH oxidase activity; but OZ-stimulated activity is independent of NADPH oxidase activity. |
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ISSN: | 0039-906X 1881-4751 |
DOI: | 10.7600/jspfsm.58.255 |