Structure of 3,6-dideoxy-3-[(R)-2-hydroxypropanoylamino]-D-galactose-containing O-polysaccharide from marine bacterium Simiduia litorea KMM 9504T

The O-polysaccharide was isolated from the lipopolysaccharide of a marine bacterium Simiduia litorea KMM 9504T and studied by chemical methods along with 1D and 2D 1H and 13C NMR spectroscopy including 1H,1H-TOCSY, 1H,1H-COSY, 1H,1H-ROESY, 1H,13C-HSQC and 1H,13C-HMBC experiments. The following new structure of the O-polysaccharide of S. litorea KMM 9504T containing D-galacturonamide, 2-acetamido-2,6-dideoxy-D-glucose and 3,6-dideoxy-3-[(R)-2-hydroxypropanoylamino]-D-galactose was established: [Display omitted] [Display omitted] •Structure of the OPS of S. litorea KMM 9504T was established.•The repeating unit of the OPS is a branched trisaccharide.•The OPS contains 3,6-dideoxy-3-[(R)-2-hydroxypropanoylamino]-D-galactose.