Activity, diversity and population size of ammonia-oxidising bacteria in oil-contaminated landfarming soil

Chemolithotrophic ammonia-oxidising bacteria (AOB) present in oil-contaminated landfarming soil were studied over two growing seasons in 1999 and 2000. The number of AOB (4–9 × 10 5 cells g −1 of dry soil) determined with the quantitative polymerase chain reaction (real-time PCR) and the rate of pot...

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Veröffentlicht in:	FEMS microbiology letters 2005-09, Vol.250 (1), p.33-38
Hauptverfasser:	Kurola, Jukka, Salkinoja-Salonen, Mirja, Aarnio, Tuula, Hultman, Jenni, Romantschuk, Martin
Format:	Artikel
Sprache:	eng
Schlagworte:	Agronomy. Soil science and plant productions Ammonia Ammonia - metabolism Ammonium Bacteria Bacteria - classification Bacteria - genetics Bacteria - isolation & purification Bacteria - metabolism Bacteriology Biochemistry and biology Biological and medical sciences Chemical, physicochemical, biochemical and biological properties DNA, Bacterial - genetics DNA, Bacterial - isolation & purification Electrophoresis Fundamental and applied biological sciences. Psychology Gel electrophoresis Gene sequencing Genes, Bacterial Genetic diversity Genetic Variation Growing season Metabolism. Enzymes Microbiology Nitrogen dioxide Nitrosomonadaceae - classification Nitrosomonadaceae - genetics Nitrosomonadaceae - isolation & purification Nitrosomonadaceae - metabolism Nitrosospira sp Oil pollution Oils - metabolism Oxidation Oxidation-Reduction Phylogeny Physics, chemistry, biochemistry and biology of agricultural and forest soils Polymerase chain reaction Population number Real-time PCR RNA, Bacterial - genetics RNA, Ribosomal, 16S - genetics rRNA 16S Soil bacteria Soil contamination Soil Microbiology Soil microorganisms Soil Pollutants - metabolism Soil pollution Soil science Soils Terrestrial environments
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Zusammenfassung:	Chemolithotrophic ammonia-oxidising bacteria (AOB) present in oil-contaminated landfarming soil were studied over two growing seasons in 1999 and 2000. The number of AOB (4–9 × 10 5 cells g −1 of dry soil) determined with the quantitative polymerase chain reaction (real-time PCR) and the rate of potential ammonium oxidation ( 0 . 05 – 0 . 28 μ g NO 2 - – N g - 1 of dry soil h - 1 ) indicated the presence of stable AOB populations. Denaturing gradient gel electrophoresis (DGGE) profiling and sequence analysis of PCR-amplified AOB 16S rRNA genes showed dominance of Nitrosospira-like sequences in clusters 2 and 3. The present results from the chronically oil-contaminated landfarming soil support the suggested importance of Nitrosospira-like AOB in terrestrial environments.
ISSN:	0378-1097 1574-6968
DOI:	10.1016/j.femsle.2005.06.057