Evaluation of the Roche LightMix Gastro parasites multiplex PCR assay detecting Giardia duodenalis, Entamoeba histolytica, cryptosporidia, Dientamoeba fragilis, and Blastocystis hominis

Multiplex PCR assays offer highly sensitive and specific tools for the detection of enteric pathogens. This prospective study aimed at comparing the novel Roche LightMix Modular Assay Gastro Parasites (LMAGP) detecting Giardia duodenalis, Entamoeba histolytica, Cryptosporidium spp., Blastocystis hom...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Clinical microbiology and infection 2018-12, Vol.24 (12), p.1333-1337
Hauptverfasser:	Friesen, J., Fuhrmann, J., Kietzmann, H., Tannich, E., Müller, M., Ignatius, R.
Format:	Artikel
Sprache:	eng
Schlagworte:	Adolescent Adult Aged Aged, 80 and over Blastocystis hominis Blastocystis hominis - genetics Blastocystis hominis - isolation & purification Child Child, Preschool Clinical Laboratory Techniques - methods Cryptosporidia Cryptosporidium - genetics Cryptosporidium - isolation & purification Dientamoeba - genetics Dientamoeba - isolation & purification Dientamoeba fragilis Entamoeba histolytica Entamoeba histolytica - genetics Entamoeba histolytica - isolation & purification Feces - parasitology Female Giardia duodenalis Giardia lamblia - genetics Giardia lamblia - isolation & purification Humans Immunoenzyme Techniques - methods Infant Infant, Newborn Intestinal Diseases, Parasitic - diagnosis Intestinal Diseases, Parasitic - parasitology Male Microscopy - methods Middle Aged Multiplex PCR Multiplex Polymerase Chain Reaction - methods Prospective Studies Reagent Kits, Diagnostic - statistics & numerical data Sensitivity and Specificity Young Adult
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	Multiplex PCR assays offer highly sensitive and specific tools for the detection of enteric pathogens. This prospective study aimed at comparing the novel Roche LightMix Modular Assay Gastro Parasites (LMAGP) detecting Giardia duodenalis, Entamoeba histolytica, Cryptosporidium spp., Blastocystis hominis, and Dientamoeba fragilis with routine laboratory procedures. Stool specimens (n = 1062 from 1009 patients) were consecutively examined by LMAGP, R-Biopharm Ridascreen enzyme immunoassays (EIAs) detecting G. duodenalis or E. histolytica/dispar, and microscopy of wet mounts. Discrepant results were analysed by in-house PCR. D. fragilis or B. hominis were detected by LMAGP in 131 (14.4%) and 179 (19.9%; 16 samples positive by microscopy; p
ISSN:	1198-743X 1469-0691
DOI:	10.1016/j.cmi.2018.03.025