Sulfated vizantin induces formation of macrophage extracellular traps
ABSTRACT Vizantin is an insoluble adjuvant that activates macrophages and lymphocytes. Recently, 2,2′,3,3′,4,4′‐hexasulfated‐vizantin (sulfated vizantin), which enables solubilization of vizantin, was developed by the present team. Sulfated vizantin was found to enhance bactericidal activity against...
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Veröffentlicht in: | Microbiology and immunology 2018-05, Vol.62 (5), p.310-316 |
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Sprache: | eng |
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Zusammenfassung: | ABSTRACT
Vizantin is an insoluble adjuvant that activates macrophages and lymphocytes. Recently, 2,2′,3,3′,4,4′‐hexasulfated‐vizantin (sulfated vizantin), which enables solubilization of vizantin, was developed by the present team. Sulfated vizantin was found to enhance bactericidal activity against multi‐drug resistant Pseudomonas aeruginosa in RAW264.7 cells. In addition, spread of P. aeruginosa was inhibited in RAW264.7 cells treated with sulfated vizantin. When only sulfated vizantin and P. aeruginosa were incubated, sulfated vizantin did not affect growth of P. aeruginosa. Formation of DNA‐based extracellular traps (ETs), a novel defense mechanism in several types of innate immune cells, helps to eliminate pathogens. In the present study, ET‐forming macrophages constituted the majority of immune cells. Sulfated vizantin induced ET formation in RAW264.7 cells, whereas a Ca‐chelating reagent, EDTA, and T‐type calcium channel blocker, tetrandrine, inhibited ET formation and attenuated inhibition of spread of P. aeruginosa in sulfated vizantin‐treated cells. Thus, sulfated vizantin induces ET formation in phagocytic cells in a Ca‐dependent manner, thus preventing spread of P. aeruginosa. Hence, sulfated vizantin may be useful in the management of infectious diseases. |
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ISSN: | 0385-5600 1348-0421 |
DOI: | 10.1111/1348-0421.12589 |