Variability of the Expression of Reference Genes in Samples of Human Olfactory Epithelium

Variability of the Expression of Reference Genes in Samples of Human Olfactory Epithelium

We analyzed variability of the expression of three reference genes in biopsy samples of the olfactory epithelium obtained from healthy volunteers. The expression of B2M , HPRT1 , and CASC3 genes was analyzed by real-time PCR. The pairs of genes B2M — HPRT1 and B2M—CASC3 were found to possess minimum...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Bulletin of experimental biology and medicine 2018-03, Vol.164 (5), p.655-657
Hauptverfasser: Kovalenko, S. P., Rudenko, N. S., Avdeeva, A. S., Danilenko, K. V., Aftanas, L. I.
Format: Artikel
Sprache:eng
Schlagworte:
Adolescent
Adult
Biomedical and Life Sciences
Biomedicine
Biopsy
Bipolar disorder
Cell Biology
Epithelium
Female
Gene expression
Gene Expression Profiling
Gene Expression Regulation, Neoplastic - genetics
Genes
Genetic aspects
Humans
Hypoxanthine Phosphoribosyltransferase - metabolism
In Vitro Techniques
Internal Medicine
Laboratory Medicine
Male
Middle Aged
Neoplasm Proteins - metabolism
Nuclear Proteins - metabolism
Olfactory epithelium
Olfactory Mucosa - metabolism
Pathology
RNA
Smell
Stem cells
Young Adult
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:We analyzed variability of the expression of three reference genes in biopsy samples of the olfactory epithelium obtained from healthy volunteers. The expression of B2M , HPRT1 , and CASC3 genes was analyzed by real-time PCR. The pairs of genes B2M — HPRT1 and B2M—CASC3 were found to possess minimum individual variability of expression and can be reliable candidates for the reference genes in analysis of gene expression in neural cells.
ISSN:0007-4888
1573-8221
DOI:10.1007/s10517-018-4052-7