Establishment of CTAB Turbidimetric method to determine hyaluronic acid content in fermentation broth

The characters of visible spectra on the formation of turbidity between hyaluronic acid (HA) and cetyltrimethylammonium bromide (CTAB) were investigated. The effects of reaction time and temperature on the formation of turbidity were also explored. Consequently, CTAB Turbidimetric method (CTM), a qu...

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Veröffentlicht in:Carbohydrate polymers 2009-08, Vol.78 (1), p.178-181
Hauptverfasser: Chen, Yong-Hao, Wang, Qiang
Format: Artikel
Sprache:eng
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Zusammenfassung:The characters of visible spectra on the formation of turbidity between hyaluronic acid (HA) and cetyltrimethylammonium bromide (CTAB) were investigated. The effects of reaction time and temperature on the formation of turbidity were also explored. Consequently, CTAB Turbidimetric method (CTM), a quick and safe method to determine HA content in large batch fermentation broth samples, was established. CTM and Bitter–Muir method (BMM) were respectively applied to measure HA content and were thoroughly compared. The results indicate that CTM was superior to BMM in terms of accuracy, precision and sensitivity. CTM is a promising alternative to BMM to determine HA content in fermentation broth.
ISSN:0144-8617
1879-1344
DOI:10.1016/j.carbpol.2009.04.037