Establishment and characterization of three cell lines derived from the rockfish grouper Epinephelus quoyanus: Use for transgenic studies and cytotoxicity testing

The establishment and partial characterization of continuous cell lines from Epinephelus quoyanus are described. Three new cell lines, designated as RGB, RGG and RGH, were established from brain, gill and heart tissue, respectively, of the rockfish grouper E. quoyanus. Since their initiation in December of 2006, these cell lines have been subcultured in Leibovitz's L-15 medium with 10% fetal bovine serum for more than 80 passages at 30 °C. The morphologies of RGB and RGG cells were epithelioid, while RGH was predominantly fibroblast-like. Propagation of these cell lines was serum-dependent, with a low plating efficiency (