Genetic inactivation of dace, Leuciscus leuciscus (L.), gametes using UV irradiation

The results of genetic inactivation of dace, Leuciscus leuciscus (L.), gametes using UV irradiation are presented. Dace sperm was genetically inactivated with UV irradiation doses ranging from 384 to 3840 J/square m. The most effective dose, expressed as a high percentage of hatched haploid larvae,...

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Veröffentlicht in:	Archives of Polish Fisheries 2008-01, Vol.16 (4), p.437-446
Hauptverfasser:	Kucharczyk, D, Targonska K.,University of Warmia and Mazury, Olsztyn (Poland). Dept. of Lake and River Fisheries, Szczerbowski, A, Luczynski, M.J.,Inland Fisheries Institute, Olsztyn (Poland). Dept. of Aquaculture, Rozek, W.,Olesnica Fish Farm (Poland), Kujawa, R, Mamcarz, A.,University of Warmia and Mazury, Olsztyn (Poland). Dept. of Lake and River Fisheries
Format:	Artikel
Sprache:	eng
Schlagworte:	ANDROGENESE ANDROGENESIS CYPRINIDAE DISORDERS ESPERMATOZOO Freshwater GENETIC ENGINEERING GENIE GENETIQUE GINOGENESIS GYNOGENESE GYNOGENESIS HAPLOIDIA HAPLOIDIE HAPLOIDY http://www.fao.org/aos/agrovoc#c_15584 http://www.fao.org/aos/agrovoc#c_15974 http://www.fao.org/aos/agrovoc#c_25421 http://www.fao.org/aos/agrovoc#c_26859 http://www.fao.org/aos/agrovoc#c_28433 http://www.fao.org/aos/agrovoc#c_3490 http://www.fao.org/aos/agrovoc#c_40064 http://www.fao.org/aos/agrovoc#c_40123 http://www.fao.org/aos/agrovoc#c_40125 http://www.fao.org/aos/agrovoc#c_5454 http://www.fao.org/aos/agrovoc#c_7287 INGENIERIA GENETICA IRRADIACION ULTRAVIOLETA IRRADIATION ULTRAVIOLETTE LEUCISCUS IDUS LEUCISCUS LEUCISCUS OOCYTES OVA OVULE OVULO SPERMATOZOA SPERMATOZOIDE TRASTORNOS TROUBLE ULTRAVIOLET IRRADIATION UV TREATMENT
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Zusammenfassung:	The results of genetic inactivation of dace, Leuciscus leuciscus (L.), gametes using UV irradiation are presented. Dace sperm was genetically inactivated with UV irradiation doses ranging from 384 to 3840 J/square m. The most effective dose, expressed as a high percentage of hatched haploid larvae, was 1920 J/square m. The UV treatment did not kill the spermatozoa, and following irradiation the activated spermatozoa exhibited a high percentage of motility. The percentage of hatched larvae in all the experimental groups, in which genetically inactivated dace eggs were fertilized with spermatozoa from the yellow form of ide, Leuciscus idus (L.), was much lower than in the control groups. All haploid larvae showed morphological abnormalities known as 'haploid syndrome'
ISSN:	1230-6428 2083-6139
DOI:	10.2478/s10086-008-0030-4