Cell-loaded carboxymethylcellulose microspheres sustain viability and proliferation of ATDC5 cells
Cell-loaded carboxymethylcellulose (CMC) microspheres were generated via a flow focusing microfluidic device, with a final aim to obtain viable ATDC5 aggregates with sustained proliferation capacity. We synthesized various CMC with phenolic groups (CMC-Ph) and demonstrated that high CMC-Ph molecular...
Gespeichert in:
| Veröffentlicht in: | Artificial cells, nanomedicine, and biotechnology nanomedicine, and biotechnology, 2018-01, Vol.46 (sup2), p.140-151 |
|---|---|
| Hauptverfasser: | , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 151 |
|---|---|
| container_issue | sup2 |
| container_start_page | 140 |
| container_title | Artificial cells, nanomedicine, and biotechnology |
| container_volume | 46 |
| creator | Ke, Yu Liu, Caikun Wang, Yanting Xiao, Meng Fan, Jiachen Fu, Pengcheng Wang, Shuhao Wu, Gang |
| description | Cell-loaded carboxymethylcellulose (CMC) microspheres were generated via a flow focusing microfluidic device, with a final aim to obtain viable ATDC5 aggregates with sustained proliferation capacity. We synthesized various CMC with phenolic groups (CMC-Ph) and demonstrated that high CMC-Ph molecular weight, high CMC-Ph concentration (>0.8 g/ml) or long culturing period had obvious inhibition effect on ATDC5 proliferation, but low horseradish peroxidase concentration (HRP, |
| doi_str_mv | 10.1080/21691401.2018.1452751 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_infor</sourceid><recordid>TN_cdi_proquest_miscellaneous_2015841211</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2183165865</sourcerecordid><originalsourceid>FETCH-LOGICAL-c441t-f578dfdacad600efa81fe8001f466451de388aa71558f8cced5124cdc51c75593</originalsourceid><addsrcrecordid>eNp9kU9P3DAQxS1UVBDsRyiyxKWXbD1ZT-K9gbaFVkLqZSv1Znn9Rxg58WIn0Hz7OtqFQw_1xZb9mzdv_Aj5BGwJTLAvNTRr4AyWNQOxBI51i3BCzuf7Cjj8_vB-ZnBGFjk_sbIENC3yj-SsXiO2pfac7DY2hCpEZayhWqVd_DN1dnicgi4PY4jZ0s7rFPP-0SabaR7zoHxPX7za-eCHiare0H2KwTub1OBjT6Ojt9uvG6SzRr4kp06FbBfH_YL8uvu23XyvHn7e_9jcPlSacxgqh60wziitTMOYdUqAs4IxcLxpOIKxKyGUagFROKG1NQg110Yj6BZxvbognw-6xczzaPMgO59nB6q3ccyyzIuCQw1Q0Ot_0Kc4pr64kzWIFTQoGiwUHqh5_Jysk_vkO5UmCUzOOci3HGZtIY85lLqro_q466x5r3r79QLcHADfu5g69RpTMHJQU4jJJdVrn-Xq_z3-AhzQl5c</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2183165865</pqid></control><display><type>article</type><title>Cell-loaded carboxymethylcellulose microspheres sustain viability and proliferation of ATDC5 cells</title><source>Alma/SFX Local Collection</source><creator>Ke, Yu ; Liu, Caikun ; Wang, Yanting ; Xiao, Meng ; Fan, Jiachen ; Fu, Pengcheng ; Wang, Shuhao ; Wu, Gang</creator><creatorcontrib>Ke, Yu ; Liu, Caikun ; Wang, Yanting ; Xiao, Meng ; Fan, Jiachen ; Fu, Pengcheng ; Wang, Shuhao ; Wu, Gang</creatorcontrib><description>Cell-loaded carboxymethylcellulose (CMC) microspheres were generated via a flow focusing microfluidic device, with a final aim to obtain viable ATDC5 aggregates with sustained proliferation capacity. We synthesized various CMC with phenolic groups (CMC-Ph) and demonstrated that high CMC-Ph molecular weight, high CMC-Ph concentration (>0.8 g/ml) or long culturing period had obvious inhibition effect on ATDC5 proliferation, but low horseradish peroxidase concentration (HRP, <0.4 mg/ml) did not. CMC-Ph gels being obtained through HRP/H
2
O
2
reaction showed an enhancing strength and decreasing break stain as the molecular weight of CMC-Ph increased, along with a decreasing gelation time. The microfluidics-based synthesis of cell-loaded microspheres with great design flexibilities was achieved using CMC-Ph with weight-average molecular weight of 1.0 × 10
5
. ATDC5 cells were viable up to 41 days of culture and proliferated gradually with increasing culture time. High cell density in CMC-Ph solution and high fetal bovine serum concentration in culture medium were prone to forming cell aggregates. Isolated cells from the microspheres showed the typical spherical morphology of undifferentiated ATDC5. Therefore, CMC-Ph microspheres might be used as cell aggregates depots to study cell-cell or cell-biomaterials functions for tissue engineering applications.</description><identifier>ISSN: 2169-1401</identifier><identifier>EISSN: 2169-141X</identifier><identifier>DOI: 10.1080/21691401.2018.1452751</identifier><identifier>PMID: 29557201</identifier><language>eng</language><publisher>England: Taylor & Francis</publisher><subject>Aggregates ; ATDC5 ; Biomaterials ; Biomedical materials ; Carboxymethylcellulose ; Cell culture ; Cell density ; Cell proliferation ; cell-loaded microspheres ; Cytology ; fast gelation ; Gelation ; Gels ; Horseradish peroxidase ; Hydrogen peroxide ; microfluidic ; Microfluidics ; Microspheres ; Molecular weight ; Morphology ; Peroxidase ; Phenolic compounds ; Tissue engineering ; Viability</subject><ispartof>Artificial cells, nanomedicine, and biotechnology, 2018-01, Vol.46 (sup2), p.140-151</ispartof><rights>2018 Informa UK Limited, trading as Taylor & Francis Group 2018</rights><rights>2018 Informa UK Limited, trading as Taylor & Francis Group</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c441t-f578dfdacad600efa81fe8001f466451de388aa71558f8cced5124cdc51c75593</citedby><cites>FETCH-LOGICAL-c441t-f578dfdacad600efa81fe8001f466451de388aa71558f8cced5124cdc51c75593</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29557201$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ke, Yu</creatorcontrib><creatorcontrib>Liu, Caikun</creatorcontrib><creatorcontrib>Wang, Yanting</creatorcontrib><creatorcontrib>Xiao, Meng</creatorcontrib><creatorcontrib>Fan, Jiachen</creatorcontrib><creatorcontrib>Fu, Pengcheng</creatorcontrib><creatorcontrib>Wang, Shuhao</creatorcontrib><creatorcontrib>Wu, Gang</creatorcontrib><title>Cell-loaded carboxymethylcellulose microspheres sustain viability and proliferation of ATDC5 cells</title><title>Artificial cells, nanomedicine, and biotechnology</title><addtitle>Artif Cells Nanomed Biotechnol</addtitle><description>Cell-loaded carboxymethylcellulose (CMC) microspheres were generated via a flow focusing microfluidic device, with a final aim to obtain viable ATDC5 aggregates with sustained proliferation capacity. We synthesized various CMC with phenolic groups (CMC-Ph) and demonstrated that high CMC-Ph molecular weight, high CMC-Ph concentration (>0.8 g/ml) or long culturing period had obvious inhibition effect on ATDC5 proliferation, but low horseradish peroxidase concentration (HRP, <0.4 mg/ml) did not. CMC-Ph gels being obtained through HRP/H
2
O
2
reaction showed an enhancing strength and decreasing break stain as the molecular weight of CMC-Ph increased, along with a decreasing gelation time. The microfluidics-based synthesis of cell-loaded microspheres with great design flexibilities was achieved using CMC-Ph with weight-average molecular weight of 1.0 × 10
5
. ATDC5 cells were viable up to 41 days of culture and proliferated gradually with increasing culture time. High cell density in CMC-Ph solution and high fetal bovine serum concentration in culture medium were prone to forming cell aggregates. Isolated cells from the microspheres showed the typical spherical morphology of undifferentiated ATDC5. Therefore, CMC-Ph microspheres might be used as cell aggregates depots to study cell-cell or cell-biomaterials functions for tissue engineering applications.</description><subject>Aggregates</subject><subject>ATDC5</subject><subject>Biomaterials</subject><subject>Biomedical materials</subject><subject>Carboxymethylcellulose</subject><subject>Cell culture</subject><subject>Cell density</subject><subject>Cell proliferation</subject><subject>cell-loaded microspheres</subject><subject>Cytology</subject><subject>fast gelation</subject><subject>Gelation</subject><subject>Gels</subject><subject>Horseradish peroxidase</subject><subject>Hydrogen peroxide</subject><subject>microfluidic</subject><subject>Microfluidics</subject><subject>Microspheres</subject><subject>Molecular weight</subject><subject>Morphology</subject><subject>Peroxidase</subject><subject>Phenolic compounds</subject><subject>Tissue engineering</subject><subject>Viability</subject><issn>2169-1401</issn><issn>2169-141X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><recordid>eNp9kU9P3DAQxS1UVBDsRyiyxKWXbD1ZT-K9gbaFVkLqZSv1Znn9Rxg58WIn0Hz7OtqFQw_1xZb9mzdv_Aj5BGwJTLAvNTRr4AyWNQOxBI51i3BCzuf7Cjj8_vB-ZnBGFjk_sbIENC3yj-SsXiO2pfac7DY2hCpEZayhWqVd_DN1dnicgi4PY4jZ0s7rFPP-0SabaR7zoHxPX7za-eCHiare0H2KwTub1OBjT6Ojt9uvG6SzRr4kp06FbBfH_YL8uvu23XyvHn7e_9jcPlSacxgqh60wziitTMOYdUqAs4IxcLxpOIKxKyGUagFROKG1NQg110Yj6BZxvbognw-6xczzaPMgO59nB6q3ccyyzIuCQw1Q0Ot_0Kc4pr64kzWIFTQoGiwUHqh5_Jysk_vkO5UmCUzOOci3HGZtIY85lLqro_q466x5r3r79QLcHADfu5g69RpTMHJQU4jJJdVrn-Xq_z3-AhzQl5c</recordid><startdate>20180101</startdate><enddate>20180101</enddate><creator>Ke, Yu</creator><creator>Liu, Caikun</creator><creator>Wang, Yanting</creator><creator>Xiao, Meng</creator><creator>Fan, Jiachen</creator><creator>Fu, Pengcheng</creator><creator>Wang, Shuhao</creator><creator>Wu, Gang</creator><general>Taylor & Francis</general><general>Taylor & Francis Ltd</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20180101</creationdate><title>Cell-loaded carboxymethylcellulose microspheres sustain viability and proliferation of ATDC5 cells</title><author>Ke, Yu ; Liu, Caikun ; Wang, Yanting ; Xiao, Meng ; Fan, Jiachen ; Fu, Pengcheng ; Wang, Shuhao ; Wu, Gang</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c441t-f578dfdacad600efa81fe8001f466451de388aa71558f8cced5124cdc51c75593</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Aggregates</topic><topic>ATDC5</topic><topic>Biomaterials</topic><topic>Biomedical materials</topic><topic>Carboxymethylcellulose</topic><topic>Cell culture</topic><topic>Cell density</topic><topic>Cell proliferation</topic><topic>cell-loaded microspheres</topic><topic>Cytology</topic><topic>fast gelation</topic><topic>Gelation</topic><topic>Gels</topic><topic>Horseradish peroxidase</topic><topic>Hydrogen peroxide</topic><topic>microfluidic</topic><topic>Microfluidics</topic><topic>Microspheres</topic><topic>Molecular weight</topic><topic>Morphology</topic><topic>Peroxidase</topic><topic>Phenolic compounds</topic><topic>Tissue engineering</topic><topic>Viability</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ke, Yu</creatorcontrib><creatorcontrib>Liu, Caikun</creatorcontrib><creatorcontrib>Wang, Yanting</creatorcontrib><creatorcontrib>Xiao, Meng</creatorcontrib><creatorcontrib>Fan, Jiachen</creatorcontrib><creatorcontrib>Fu, Pengcheng</creatorcontrib><creatorcontrib>Wang, Shuhao</creatorcontrib><creatorcontrib>Wu, Gang</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Artificial cells, nanomedicine, and biotechnology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ke, Yu</au><au>Liu, Caikun</au><au>Wang, Yanting</au><au>Xiao, Meng</au><au>Fan, Jiachen</au><au>Fu, Pengcheng</au><au>Wang, Shuhao</au><au>Wu, Gang</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cell-loaded carboxymethylcellulose microspheres sustain viability and proliferation of ATDC5 cells</atitle><jtitle>Artificial cells, nanomedicine, and biotechnology</jtitle><addtitle>Artif Cells Nanomed Biotechnol</addtitle><date>2018-01-01</date><risdate>2018</risdate><volume>46</volume><issue>sup2</issue><spage>140</spage><epage>151</epage><pages>140-151</pages><issn>2169-1401</issn><eissn>2169-141X</eissn><abstract>Cell-loaded carboxymethylcellulose (CMC) microspheres were generated via a flow focusing microfluidic device, with a final aim to obtain viable ATDC5 aggregates with sustained proliferation capacity. We synthesized various CMC with phenolic groups (CMC-Ph) and demonstrated that high CMC-Ph molecular weight, high CMC-Ph concentration (>0.8 g/ml) or long culturing period had obvious inhibition effect on ATDC5 proliferation, but low horseradish peroxidase concentration (HRP, <0.4 mg/ml) did not. CMC-Ph gels being obtained through HRP/H
2
O
2
reaction showed an enhancing strength and decreasing break stain as the molecular weight of CMC-Ph increased, along with a decreasing gelation time. The microfluidics-based synthesis of cell-loaded microspheres with great design flexibilities was achieved using CMC-Ph with weight-average molecular weight of 1.0 × 10
5
. ATDC5 cells were viable up to 41 days of culture and proliferated gradually with increasing culture time. High cell density in CMC-Ph solution and high fetal bovine serum concentration in culture medium were prone to forming cell aggregates. Isolated cells from the microspheres showed the typical spherical morphology of undifferentiated ATDC5. Therefore, CMC-Ph microspheres might be used as cell aggregates depots to study cell-cell or cell-biomaterials functions for tissue engineering applications.</abstract><cop>England</cop><pub>Taylor & Francis</pub><pmid>29557201</pmid><doi>10.1080/21691401.2018.1452751</doi><tpages>12</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 2169-1401 |
| ispartof | Artificial cells, nanomedicine, and biotechnology, 2018-01, Vol.46 (sup2), p.140-151 |
| issn | 2169-1401 2169-141X |
| language | eng |
| recordid | cdi_proquest_miscellaneous_2015841211 |
| source | Alma/SFX Local Collection |
| subjects | Aggregates ATDC5 Biomaterials Biomedical materials Carboxymethylcellulose Cell culture Cell density Cell proliferation cell-loaded microspheres Cytology fast gelation Gelation Gels Horseradish peroxidase Hydrogen peroxide microfluidic Microfluidics Microspheres Molecular weight Morphology Peroxidase Phenolic compounds Tissue engineering Viability |
| title | Cell-loaded carboxymethylcellulose microspheres sustain viability and proliferation of ATDC5 cells |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-14T15%3A53%3A44IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_infor&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Cell-loaded%20carboxymethylcellulose%20microspheres%20sustain%20viability%20and%20proliferation%20of%20ATDC5%20cells&rft.jtitle=Artificial%20cells,%20nanomedicine,%20and%20biotechnology&rft.au=Ke,%20Yu&rft.date=2018-01-01&rft.volume=46&rft.issue=sup2&rft.spage=140&rft.epage=151&rft.pages=140-151&rft.issn=2169-1401&rft.eissn=2169-141X&rft_id=info:doi/10.1080/21691401.2018.1452751&rft_dat=%3Cproquest_infor%3E2183165865%3C/proquest_infor%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2183165865&rft_id=info:pmid/29557201&rfr_iscdi=true |