Identification and characterization of two novel Gammapapillomavirus genomes in skin of an immunosuppressed Epidermodysplasia Verruciformis patient

•Two novel gamma-HPV genomes were isolated and cloned from the skin of an EV-patient.•The L1 ORF of HPV_MTS3 was 77% similar to its closest HPV isolate w18c39.•The L1 ORF of HPV_MTS4 was 91% similar to its closest HPV isolate EV03c60.•The novel HPV_MTS3 and HPV_MTS4 belong to species gamma-22 and gamma-7, respectively. Two novel human gamma-papillomavirus genomes (HPV_MTS3, and HPV_MTS4) were isolated from the skin of an immunosuppressed, late-onset Epidermodysplasia Verruciformis patient and fully cloned. The L1 open reading frames of HPV_MTS3 and HPV_MTS4 were 77% and 91% identical to their closest HPV full genome isolates w18c39 and EV03c60, which belong to the species gamma-22and gamma-7 of the genus Gammapapillomavirus, respectively.