Acetone soluble mutagenicity assessment tested by a salmonella/microsome system (AMES) in the Strait of Marmara (Black Sea and the Sea of Marmara)

Many researches have been conducted on various parameters related to the pollution in the Black Sea, the Sea of Marmara and the Strait of Istanbul. However, there is no report on the mutagenicity tested by evaluating the mutagenic effects of the total pollution, dissolving water samples in acetone, and using a salmonella/ microsome test system. It is known that the increasing pollutant load of the Black Sea, the Sea of Marmara, and the Strait of Istanbul, are especially due to sea-traffic and out-regional factors drifted. It is important to assess the mutagenic effect of pollution on DNA reflecting the changes in functional and structural proteins of the organisms exposed. Water samples were collected at 20 stations of the Strait of Istanbul, evaporated, dissolved in acetone and evaluated by AMES test using two different test strains, Salmonella typhimurium TA98 and TA100. Mutagenic effects have been detected for the stations Eminoenue, Kasimpasa, Balat, and Eyuep. Only weak mutagenicity has been observed for the stations Besiktas, Ueskuedar, Pasabahce, Cayirbasi, Sahilyolu and Kadikoey.