Purification and functional characterization of recombinant balsamin, a ribosome-inactivating protein from Momordica balsamina

Balsamin, a type I ribosome-inactivating protein (RIP), has been shown to inhibit HIV-1 replication at the translation step. Our recent studies have shown that balsamin also possess anti-tumor, antibacterial and DNase-like activity, however, the amount of natural balsamin in Momordica balsamina seeds is limited and preclinical studies require large quantities of pure, bioactive balsamin. Therefore, in this study, we cloned the balsamin gene, expressed it in E.coli BL21 (DE3) strain and purified it by nickel affinity chromatography. Functional analysis indicated that balsamin exhibits both RNA N-glycosidase activity, releasing the Endo-fragment from rabbit reticulocyte rRNA, and DNase-like activity, converting the supercoiled form of a plasmid into the linear form in a concentration-dependent manner. Analysis of secondary structure revealed that recombinant balsamin mainly consisted of α-helical and random coiled with minimal turns and β-sheets. Recombinant balsamin was found to be stable in the temperature range of 20–60 °C and pH range of 6–9. Antimicrobial assays showed that the minimum inhibitory concentrations of recombinant balsamin for various pathogens ranged between 1.56 and 12.5 μg/ml. Heterologous expression and purification of balsamin carries great importance as it provides an alternative approach for large-scale preparation of biologically active recombinant balsamin, which is difficult from its natural source. •Balsamin was cloned and expressed in E.coli BL21 (DE3) strain, and purified by two-step purification.•Recombinant balsamin exhibited RNA N-glycosidase and DNase-like activity.•rBalsamin consisted of α-helical and random coiled with minimal turns and β-sheets.•Structure of rBalsamin was found to be sensitive to higher temperatures, acidic/basic environment.•Antimicrobial assay revealed that rBalsamin inhibited the growth of various bacterial pathogens in a dose dependent manner.