Identification of microbial species present in a pesticide dissipation process in biobed systems using typical substrates from southeastern Mexico as a biomixture at a laboratory scale

•Microbial diversity in biobed systems after pesticide dissipation was studied.•>99% of the initial concentration of pesticides was dissipated at 41days.•Species of archaea (23), bacteria (598) & fungi (64) were identified in biomixtures.•Biomixture type was significant on residual pesticides and microbial diversity.•Microbial diversity and richness were significant on residual pesticides detected. [Display omitted] Biobed systems are an important option to control point pollution in agricultural areas. Substrates used and microbial diversity present in a biomixture perform an essential function in pesticide dissipation. In this study, the effects of soil (50% of volume/volume [V/V] proportion for all biomixtures) and four soil-based biomixtures (miniaturized biobeds; addition of novel substrates from southeastern Mexico) on dissipation of high concentrations of 2,4-dichlorophenoxyacetic acid (2,4-D), atrazine, carbofuran, diazinon, and glyphosate and on microbial diversity in biomixtures were evaluated. Small residual amounts of all pesticides at 20 (