Cellular toxicity driven by high-dose vitamin C on normal and cancer stem cells

As a powerful antioxidant, vitamin C protects cells from oxidative damage by inhibiting production of free radicals. However, high levels of vitamin C shows cytotoxicity especially on cancerous cells through generating excessive ROS and blocking the energy homeostasis. Although the double-sided char...

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Veröffentlicht in:Biochemical and biophysical research communications 2018-02, Vol.497 (1), p.347-353
Hauptverfasser: Kim, Tae-Jun, Byun, Jin-Seok, Kwon, Hyun Sook, Kim, Do-Yeon
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Sprache:eng
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Zusammenfassung:As a powerful antioxidant, vitamin C protects cells from oxidative damage by inhibiting production of free radicals. However, high levels of vitamin C shows cytotoxicity especially on cancerous cells through generating excessive ROS and blocking the energy homeostasis. Although the double-sided character of vitamin C has been extensively studied in many cell types, there is little research on the consequence of vitamin C treatment in stem cells. Here, we identified that high-dose vitamin C shows cellular toxicity on proliferating NSPCs. We also demonstrated that undifferentiated NSPCs are more sensitive to vitamin C-driven DNA damage than differentiated cells, due to higher expression of Glut genes. Finally, we showed that high-dose vitamin C selectively induces DNA damage on cancer stem cells rather than differentiated tumor cells, raising a possibility that vitamin C may be used to target cancer stem cells. •High-dose vitamin C induces DNA damage and apoptosis on NSPCs.•Undifferentiated NSPCs are more sensitive to vitamin C-driven DNA damage than differentiated cells.•Undifferentiated NSPCs show higher expression of Glut genes than differentiated cells.•High-dose vitamin C selectively induces DNA damage on cancer stem cells.
ISSN:0006-291X
1090-2104
DOI:10.1016/j.bbrc.2018.02.083