Radiotherapy alters the composition, structural and mechanical properties of root dentin in vitro
Objectives Post-radiation dental lesions affect mainly the cervical area of the tooth. Until now, there are quite few evidences regarding the effects of radiation exposure on root dentin breakdown. To better understand this effect, we used human root dentin specimens obtained from third molars from...
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| Veröffentlicht in: | Clinical oral investigations 2018-11, Vol.22 (8), p.2871-2878 |
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| creator | Velo, Marilia Mattar de Amoêdo Campos Farha, Ana Laura Herrera da Silva Santos, Paulo Sérgio Shiota, Aymée Sansavino, Simone Zuquerato Souza, Ana Tarsila Honório, Heitor Marques Wang, Linda |
| description | Objectives
Post-radiation dental lesions affect mainly the cervical area of the tooth. Until now, there are quite few evidences regarding the effects of radiation exposure on root dentin breakdown. To better understand this effect, we used human root dentin specimens obtained from third molars from similarly aged individuals.
Materials and methods
Twenty specimens were analyzed by the surface hardness (SH), energy-dispersive X-ray spectroscopy (EDX), and X-ray diffraction (XRD) to evaluate the baseline properties of their root dentin. Other six human teeth were prepared and analyzed by scanning electron microscopy (SEM). Then the specimens were randomly distributed between two groups (
n
= 13 per group) and irradiated with a total dose of 55 or 70 Gy in a linear accelerator. The percentage of EDX and surface hardness loss (%SHL) were determined based on measurements before and after irradiation. The specimens were also analyzed after irradiation by SEM and XRD. The Ca/P weight ratio was calculated.
Results
Based on SEM analysis, radiation exposure induced dehydration of the dentin. The Ca/P weight ratio decreased (
p
= 0.0045). The %SHL of specimens irradiated with 70 Gy was higher than that of the 55-Gy group (
p
|
| doi_str_mv | 10.1007/s00784-018-2373-6 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2001406789</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2001406789</sourcerecordid><originalsourceid>FETCH-LOGICAL-c372t-c40f2f2fdd8c2603ee9c05c7e282734cb4b36209c0e603b0b53f75c25bca4153</originalsourceid><addsrcrecordid>eNp1kNtKxDAQhoMo7np4AG8k4I0XVnNok_ZSxBMsCLL3IU2nGmmbmqTCvr3ZXQ8gSEKSyXzzZ_IjdELJJSVEXoW0lHlGaJkxLnkmdtCc5lxkXEq6uzmzTFQlnaGDEN4IobmQfB_NWJVzIiidI_2sG-viK3g9rrDuIviAU4iN60cXbLRuuMAh-snEyesO66HBPZhXPViTwtG7EXy0ELBrsXcu4gaGaAec5oeN3h2hvVZ3AY6_9kO0vLtd3jxki6f7x5vrRWa4ZDEzOWlZGk1TGiYIB6gMKYwEVjLJc1PnNReMpEtI2ZrUBW9lYVhRG53Tgh-i861s6uh9ghBVb4OBrtMDuCkotv49EbKsEnr2B31zkx9Sc2uKVJzJjSDdUsa7EDy0avS2136lKFFr-9XWfpXsV2v7lUg1p1_KU91D81Px7XcC2BYIKTW8gP99-n_VT3XEkGk</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2000932715</pqid></control><display><type>article</type><title>Radiotherapy alters the composition, structural and mechanical properties of root dentin in vitro</title><source>MEDLINE</source><source>SpringerNature Journals</source><creator>Velo, Marilia Mattar de Amoêdo Campos ; Farha, Ana Laura Herrera ; da Silva Santos, Paulo Sérgio ; Shiota, Aymée ; Sansavino, Simone Zuquerato ; Souza, Ana Tarsila ; Honório, Heitor Marques ; Wang, Linda</creator><creatorcontrib>Velo, Marilia Mattar de Amoêdo Campos ; Farha, Ana Laura Herrera ; da Silva Santos, Paulo Sérgio ; Shiota, Aymée ; Sansavino, Simone Zuquerato ; Souza, Ana Tarsila ; Honório, Heitor Marques ; Wang, Linda</creatorcontrib><description>Objectives
Post-radiation dental lesions affect mainly the cervical area of the tooth. Until now, there are quite few evidences regarding the effects of radiation exposure on root dentin breakdown. To better understand this effect, we used human root dentin specimens obtained from third molars from similarly aged individuals.
Materials and methods
Twenty specimens were analyzed by the surface hardness (SH), energy-dispersive X-ray spectroscopy (EDX), and X-ray diffraction (XRD) to evaluate the baseline properties of their root dentin. Other six human teeth were prepared and analyzed by scanning electron microscopy (SEM). Then the specimens were randomly distributed between two groups (
n
= 13 per group) and irradiated with a total dose of 55 or 70 Gy in a linear accelerator. The percentage of EDX and surface hardness loss (%SHL) were determined based on measurements before and after irradiation. The specimens were also analyzed after irradiation by SEM and XRD. The Ca/P weight ratio was calculated.
Results
Based on SEM analysis, radiation exposure induced dehydration of the dentin. The Ca/P weight ratio decreased (
p
= 0.0045). The %SHL of specimens irradiated with 70 Gy was higher than that of the 55-Gy group (
p
< 0.05), although even the lower dose induced root dentin breakdown.
Conclusions
Overall, we can state that radiation exposure changes the composition and structure of human root dentin, which detrimentally affect its hardness.
Clinical relevance
The changes reported herein might influence the selection of the dental materials and will bring new knowledge in this field to prevent radiation-related caries in root dentin.</description><identifier>ISSN: 1432-6981</identifier><identifier>EISSN: 1436-3771</identifier><identifier>DOI: 10.1007/s00784-018-2373-6</identifier><identifier>PMID: 29430611</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Dehydration ; Dental caries ; Dentin ; Dentin - chemistry ; Dentin - radiation effects ; Dentistry ; Hardness ; Humans ; In Vitro Techniques ; Mechanical properties ; Medicine ; Microscopy, Electron, Scanning ; Molar, Third ; Molars ; Original Article ; Post-radiation ; Radiation therapy ; Scanning electron microscopy ; Spectrometry, X-Ray Emission ; Teeth ; Tooth Root - radiation effects ; X-Ray Diffraction ; X-ray spectroscopy ; X-rays</subject><ispartof>Clinical oral investigations, 2018-11, Vol.22 (8), p.2871-2878</ispartof><rights>Springer-Verlag GmbH Germany, part of Springer Nature 2018</rights><rights>Clinical Oral Investigations is a copyright of Springer, (2018). All Rights Reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c372t-c40f2f2fdd8c2603ee9c05c7e282734cb4b36209c0e603b0b53f75c25bca4153</citedby><cites>FETCH-LOGICAL-c372t-c40f2f2fdd8c2603ee9c05c7e282734cb4b36209c0e603b0b53f75c25bca4153</cites><orcidid>0000-0001-6308-2769</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00784-018-2373-6$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00784-018-2373-6$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>315,782,786,27931,27932,41495,42564,51326</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29430611$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Velo, Marilia Mattar de Amoêdo Campos</creatorcontrib><creatorcontrib>Farha, Ana Laura Herrera</creatorcontrib><creatorcontrib>da Silva Santos, Paulo Sérgio</creatorcontrib><creatorcontrib>Shiota, Aymée</creatorcontrib><creatorcontrib>Sansavino, Simone Zuquerato</creatorcontrib><creatorcontrib>Souza, Ana Tarsila</creatorcontrib><creatorcontrib>Honório, Heitor Marques</creatorcontrib><creatorcontrib>Wang, Linda</creatorcontrib><title>Radiotherapy alters the composition, structural and mechanical properties of root dentin in vitro</title><title>Clinical oral investigations</title><addtitle>Clin Oral Invest</addtitle><addtitle>Clin Oral Investig</addtitle><description>Objectives
Post-radiation dental lesions affect mainly the cervical area of the tooth. Until now, there are quite few evidences regarding the effects of radiation exposure on root dentin breakdown. To better understand this effect, we used human root dentin specimens obtained from third molars from similarly aged individuals.
Materials and methods
Twenty specimens were analyzed by the surface hardness (SH), energy-dispersive X-ray spectroscopy (EDX), and X-ray diffraction (XRD) to evaluate the baseline properties of their root dentin. Other six human teeth were prepared and analyzed by scanning electron microscopy (SEM). Then the specimens were randomly distributed between two groups (
n
= 13 per group) and irradiated with a total dose of 55 or 70 Gy in a linear accelerator. The percentage of EDX and surface hardness loss (%SHL) were determined based on measurements before and after irradiation. The specimens were also analyzed after irradiation by SEM and XRD. The Ca/P weight ratio was calculated.
Results
Based on SEM analysis, radiation exposure induced dehydration of the dentin. The Ca/P weight ratio decreased (
p
= 0.0045). The %SHL of specimens irradiated with 70 Gy was higher than that of the 55-Gy group (
p
< 0.05), although even the lower dose induced root dentin breakdown.
Conclusions
Overall, we can state that radiation exposure changes the composition and structure of human root dentin, which detrimentally affect its hardness.
Clinical relevance
The changes reported herein might influence the selection of the dental materials and will bring new knowledge in this field to prevent radiation-related caries in root dentin.</description><subject>Dehydration</subject><subject>Dental caries</subject><subject>Dentin</subject><subject>Dentin - chemistry</subject><subject>Dentin - radiation effects</subject><subject>Dentistry</subject><subject>Hardness</subject><subject>Humans</subject><subject>In Vitro Techniques</subject><subject>Mechanical properties</subject><subject>Medicine</subject><subject>Microscopy, Electron, Scanning</subject><subject>Molar, Third</subject><subject>Molars</subject><subject>Original Article</subject><subject>Post-radiation</subject><subject>Radiation therapy</subject><subject>Scanning electron microscopy</subject><subject>Spectrometry, X-Ray Emission</subject><subject>Teeth</subject><subject>Tooth Root - radiation effects</subject><subject>X-Ray Diffraction</subject><subject>X-ray spectroscopy</subject><subject>X-rays</subject><issn>1432-6981</issn><issn>1436-3771</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp1kNtKxDAQhoMo7np4AG8k4I0XVnNok_ZSxBMsCLL3IU2nGmmbmqTCvr3ZXQ8gSEKSyXzzZ_IjdELJJSVEXoW0lHlGaJkxLnkmdtCc5lxkXEq6uzmzTFQlnaGDEN4IobmQfB_NWJVzIiidI_2sG-viK3g9rrDuIviAU4iN60cXbLRuuMAh-snEyesO66HBPZhXPViTwtG7EXy0ELBrsXcu4gaGaAec5oeN3h2hvVZ3AY6_9kO0vLtd3jxki6f7x5vrRWa4ZDEzOWlZGk1TGiYIB6gMKYwEVjLJc1PnNReMpEtI2ZrUBW9lYVhRG53Tgh-i861s6uh9ghBVb4OBrtMDuCkotv49EbKsEnr2B31zkx9Sc2uKVJzJjSDdUsa7EDy0avS2136lKFFr-9XWfpXsV2v7lUg1p1_KU91D81Px7XcC2BYIKTW8gP99-n_VT3XEkGk</recordid><startdate>20181101</startdate><enddate>20181101</enddate><creator>Velo, Marilia Mattar de Amoêdo Campos</creator><creator>Farha, Ana Laura Herrera</creator><creator>da Silva Santos, Paulo Sérgio</creator><creator>Shiota, Aymée</creator><creator>Sansavino, Simone Zuquerato</creator><creator>Souza, Ana Tarsila</creator><creator>Honório, Heitor Marques</creator><creator>Wang, Linda</creator><general>Springer Berlin Heidelberg</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0001-6308-2769</orcidid></search><sort><creationdate>20181101</creationdate><title>Radiotherapy alters the composition, structural and mechanical properties of root dentin in vitro</title><author>Velo, Marilia Mattar de Amoêdo Campos ; Farha, Ana Laura Herrera ; da Silva Santos, Paulo Sérgio ; Shiota, Aymée ; Sansavino, Simone Zuquerato ; Souza, Ana Tarsila ; Honório, Heitor Marques ; Wang, Linda</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c372t-c40f2f2fdd8c2603ee9c05c7e282734cb4b36209c0e603b0b53f75c25bca4153</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Dehydration</topic><topic>Dental caries</topic><topic>Dentin</topic><topic>Dentin - chemistry</topic><topic>Dentin - radiation effects</topic><topic>Dentistry</topic><topic>Hardness</topic><topic>Humans</topic><topic>In Vitro Techniques</topic><topic>Mechanical properties</topic><topic>Medicine</topic><topic>Microscopy, Electron, Scanning</topic><topic>Molar, Third</topic><topic>Molars</topic><topic>Original Article</topic><topic>Post-radiation</topic><topic>Radiation therapy</topic><topic>Scanning electron microscopy</topic><topic>Spectrometry, X-Ray Emission</topic><topic>Teeth</topic><topic>Tooth Root - radiation effects</topic><topic>X-Ray Diffraction</topic><topic>X-ray spectroscopy</topic><topic>X-rays</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Velo, Marilia Mattar de Amoêdo Campos</creatorcontrib><creatorcontrib>Farha, Ana Laura Herrera</creatorcontrib><creatorcontrib>da Silva Santos, Paulo Sérgio</creatorcontrib><creatorcontrib>Shiota, Aymée</creatorcontrib><creatorcontrib>Sansavino, Simone Zuquerato</creatorcontrib><creatorcontrib>Souza, Ana Tarsila</creatorcontrib><creatorcontrib>Honório, Heitor Marques</creatorcontrib><creatorcontrib>Wang, Linda</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Biological Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><jtitle>Clinical oral investigations</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Velo, Marilia Mattar de Amoêdo Campos</au><au>Farha, Ana Laura Herrera</au><au>da Silva Santos, Paulo Sérgio</au><au>Shiota, Aymée</au><au>Sansavino, Simone Zuquerato</au><au>Souza, Ana Tarsila</au><au>Honório, Heitor Marques</au><au>Wang, Linda</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Radiotherapy alters the composition, structural and mechanical properties of root dentin in vitro</atitle><jtitle>Clinical oral investigations</jtitle><stitle>Clin Oral Invest</stitle><addtitle>Clin Oral Investig</addtitle><date>2018-11-01</date><risdate>2018</risdate><volume>22</volume><issue>8</issue><spage>2871</spage><epage>2878</epage><pages>2871-2878</pages><issn>1432-6981</issn><eissn>1436-3771</eissn><abstract>Objectives
Post-radiation dental lesions affect mainly the cervical area of the tooth. Until now, there are quite few evidences regarding the effects of radiation exposure on root dentin breakdown. To better understand this effect, we used human root dentin specimens obtained from third molars from similarly aged individuals.
Materials and methods
Twenty specimens were analyzed by the surface hardness (SH), energy-dispersive X-ray spectroscopy (EDX), and X-ray diffraction (XRD) to evaluate the baseline properties of their root dentin. Other six human teeth were prepared and analyzed by scanning electron microscopy (SEM). Then the specimens were randomly distributed between two groups (
n
= 13 per group) and irradiated with a total dose of 55 or 70 Gy in a linear accelerator. The percentage of EDX and surface hardness loss (%SHL) were determined based on measurements before and after irradiation. The specimens were also analyzed after irradiation by SEM and XRD. The Ca/P weight ratio was calculated.
Results
Based on SEM analysis, radiation exposure induced dehydration of the dentin. The Ca/P weight ratio decreased (
p
= 0.0045). The %SHL of specimens irradiated with 70 Gy was higher than that of the 55-Gy group (
p
< 0.05), although even the lower dose induced root dentin breakdown.
Conclusions
Overall, we can state that radiation exposure changes the composition and structure of human root dentin, which detrimentally affect its hardness.
Clinical relevance
The changes reported herein might influence the selection of the dental materials and will bring new knowledge in this field to prevent radiation-related caries in root dentin.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><pmid>29430611</pmid><doi>10.1007/s00784-018-2373-6</doi><tpages>8</tpages><orcidid>https://orcid.org/0000-0001-6308-2769</orcidid></addata></record> |
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| ispartof | Clinical oral investigations, 2018-11, Vol.22 (8), p.2871-2878 |
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| source | MEDLINE; SpringerNature Journals |
| subjects | Dehydration Dental caries Dentin Dentin - chemistry Dentin - radiation effects Dentistry Hardness Humans In Vitro Techniques Mechanical properties Medicine Microscopy, Electron, Scanning Molar, Third Molars Original Article Post-radiation Radiation therapy Scanning electron microscopy Spectrometry, X-Ray Emission Teeth Tooth Root - radiation effects X-Ray Diffraction X-ray spectroscopy X-rays |
| title | Radiotherapy alters the composition, structural and mechanical properties of root dentin in vitro |
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