Effects of bacterial activity on estrogen removal in nitrifying activated sludge

The effects of bacterial activity on the degradation of estrone (E1), estradiol (E2), estriol (E3) and ethinylestradiol (EE2) in nitrifying activated sludge (NAS) were studied with different substrates and organic loading rates (OLRs) and low temperature conditions. Heterotroph was shown to have utilized glucose prior to E1 for metabolism. The co-metabolism of ammonia oxidizing bacteria (AOB) dominated the degradation of E1, E2 and EE2 in NAS. The higher the organic loading, the higher the rate of organic matter transformation, with less ammonia oxidation and less degradation of E1, E2 and EE2. The degradation of E3 in NAS was shown to be largely due to heterotroph metabolism. On the basis of the difference of apparent activity between heterotroph and AOB at 4 °C, the process of estrogen degradation via the co-metabolism of AOB was able to be identified.