Haploid parthenotes express differential response to in vitro exposure of ammonia compared to normally fertilized embryos

In the present study, we assessed whether absence of paternal genome imparts any differential response in embryos to chemical stress such as ammonia. Parthenogenesis was induced in MII stage oocytes using 10 mM SrCl2 in M16 medium. Parthenotes and normally fertilized embryos at 2 cell stage were exp...

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Veröffentlicht in:	Biochemical and biophysical research communications 2017-04, Vol.486 (1), p.88-93
Hauptverfasser:	Nair, Ramya, Mutalik, Srinivas, Dasappa, Jagadeesh Prasad, Kalthur, Guruprasad, Adiga, Satish Kumar
Format:	Artikel
Sprache:	eng
Schlagworte:	Ammonia Ammonia - pharmacology Animals Apoptosis Apoptosis - drug effects Apoptosis - genetics blastocyst Blastocyst - cytology Blastocyst - metabolism cytochrome c Diploidy Embryo, Mammalian - cytology Embryo, Mammalian - metabolism Female Fertilization Gene Expression Regulation, Developmental - drug effects genome Haploidy ion exchange Male Mice Microscopy, Fluorescence mitochondria Mitochondria - drug effects Mitochondria - metabolism Nanog Homeobox Protein - genetics Nanog Homeobox Protein - metabolism Octamer Transcription Factor-3 - genetics Octamer Transcription Factor-3 - metabolism oocytes Oocytes - cytology Oocytes - drug effects Oocytes - metabolism Parthenogenesis Parthenogenesis - genetics Reverse Transcriptase Polymerase Chain Reaction Sodium-Potassium-Exchanging ATPase - genetics Sodium-Potassium-Exchanging ATPase - metabolism Stress response stress tolerance
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Zusammenfassung:	In the present study, we assessed whether absence of paternal genome imparts any differential response in embryos to chemical stress such as ammonia. Parthenogenesis was induced in MII stage oocytes using 10 mM SrCl2 in M16 medium. Parthenotes and normally fertilized embryos at 2 cell stage were exposed to different concentrations of ammonia and cultured till blastocyst. Exposure of ammonia to normally fertilized embryos resulted in significant decrease in the developmental potential (p
ISSN:	0006-291X 1090-2104
DOI:	10.1016/j.bbrc.2017.03.004