Microarray Analysis of Bisphenol A‐induced Changes in Gene Expression in Human Oral Epithelial Cells

Bisphenol A (BPA) is a common ingredient in dental materials. However, its potential adverse effects on the oral cavity are unknown. The purpose of this study is to identify the genes responding to BPA in a human oral epithelial cell line using DNA microarray. Of the 10,368 genes examined, changes in mRNA levels were detected in seven genes: five were up‐regulated and two were down‐regulated. The expression levels of the calcium channel, voltage‐dependent, L‐type, alpha 1C subunit (CACNA1C), cell death activator CIDE‐3 (CIDE‐3), haptoglobin‐related protein (HPR), importin 4 (IPO4), and POU domain, class 2 and transcription factor 3 (POU2F3) were significantly up‐regulated in the cells exposed to 100 μM BPA. The spermatogenesis‐associated, serine‐rich 2 (S PATS2) and HSPC049 protein (HSPC049) were significantly down‐regulated. The detailed knowledge of the changes in gene expression obtained using microarray technology will provide a basis for further elucidating the molecular mechanisms of the toxic effects of BPA in the oral cavity.